Regardless of the method used to collect volatile organic residues from mainstream cigarette smoke, if Gas Chromatography (GC) is used for separation and detection, inlet conditions dramatically affect the instrument response. This effect becomes significant when multiple analytes of dramatically different chemistries are determined in a single analysis. This study examines the effect of varying the GC inlet conditions on 18 selected volatile organic residues gathered from a cryo-impinger collection method. Comparison of the data and conclusions are presented.