Extraction techniques using microwave-assisted extraction (MAE) and Soxhlet extraction (SE) are evaluated for determination of Dithiocarbamate fungicide (DTCs) residues. The concentration of CS2 in both extracts are analyzed by gas chromatography using a flame photometric detector (GC-FPD) without any treatment. MAE was compared with SE for determination of DTCs residues in tobacco. Matrix-matched calibration was necessary for reducing matrix effect in MAE, while the calibration in SE was solvent-based. Recoveries on CS2 fortified in three types of tobaccos (Burley, flue-cured and Oriental) by both MAE and SE coupled to GC-FPD analysis were 70-120% with relative standard deviation values <20%. The comparable results expressed as CS₂ on tobaccos with naturally incurred DTCs were obtained by both MAE and SE. Limit of quantifications (LOQs) were 0.50 mg/kg in both methods. SE contained some complicated and carefully-handed steps, while MAE was a simple and easy method with a single step. Extraction time per one sample in MAE was above half of that in SE. Moreover, SE needed much amount of some harmful solvents; i.e. hydrochloric acid, sodium hydroxyl and sulfuric acid, although usage of hydrochloric acid per one sample could reduce to 28% in MAE, and MAE did not need sodium hydroxyl and sulfuric acid. MAE was considered as a more effective and feasible method than SE. By introducing MAE technique, the determination of DTCs residue in tobacco could be much improved.