The sample pretreatment in determining pectin in tobacco by enzymolysis-flowanalysis method was improved in order to accelerate the analytical process. The sample was directly extracted with 5% acetic acid instead of 80% ethanol reflux extraction to remove soluble sugar from tobacco, then analyzed by enzymolysis-flowanalysis. The pectin contents in 20 flue-cured tobacco leaf samples extracted with 5% acetic acid and 80% ethanol respectively were determined. The results indicated that the average recovery of pectin by acetic acid extraction was from 99.5% to 105.7% with the CV of 2.08%. The pectin content determined with acetic acid treatment was slightly higher than that with 80% ethanol, however, the difference was not significant.