Influence of the origin of the cigarette paper cellulose on the smoke chemical composition and "in vitro" toxicity tests on mainstream cigarette smoke
Nowadays, cigarette paper is made out of cellulose coming from different sources. Wood cellulose is currently very often used, but cellulose coming from different textile plants like hemp, flax, etc. were used primarily in the past and are still present in commercial cigarette papers. This study was undertaken to determine if the origin of the cigarette paper cellulose has an impact on the chemical composition of mainstream smoke and on the results of classical in vitro toxicity tests performed on smoke. Two different cigarette models, including different blends and designs were used. In both cases, cigarettes were produced with cigarette papers made out of either pure wood cellulose, or pure plant cellulose or a mixture of wood and plant cellulose. All Hoffmann analytes with the exception of trace metals were measured in the smoke of these cigarettes. The B[a]P determination was completed with qualifications of others PAHs: Pyrene and Dibenzopyrenes. The three in vitro toxicity tests recommended by CORESTA were also performed namely: Ames and micronucleus assay on TPM; neutral red uptake test on both gas and TPM phases. The cell-free "GSH depletion" test was also used to assess the toxicity of the gaseous phase. After statistical treatment, no significant differences were found between terms of comparison for all Hoffmann analytes and with the in vitro tests. The origin of the cigarette paper cellulose was not demonstrated to have an influence on the properties of mainstream cigarette smoke.