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CORESTA Congress, Kyoto, 2004, PPOST 09

Investigation of alloplasmic tobacco lines by certain economically indices

NIKOVA V.; IANTCHEVA A.M.; PANDEVA R.; PETKOVA A.
Bulgarian Academy of Science, Institute of Genetics, Sofia, Bulgaria.

Cytoplasmic male sterile (CMS) sources with cytoplasm of Nicotiana africana( afr), N.amplexicaulis (amp), N. benthamiana (ben), N. excelsior (exc), N. longiflora (lon), N. maritima (mar), N. paniculata (pan), N. rustica (rus), N. velutina (vel) and a nucleus of N. tabacum were created through interspecific hybridization. They were recurrently backcrossed with cv. Harmanliiska basma 163 (HB) to develop alloplasmic lines. The obtained CMS analogues preserved genetically controlled morphological features of the flowers, specific for every cytoplasm. Comparative morphological and technological characterization of each alloplasmic line as well as of the fertile control (HB 163) was carried out. Most of the alloplasmic lines developed better than HB 163. CMS plants have 123,3-146,0 cm stem height against 123,4 cm and 28-34 leaves against 28 ones compared with the fertile control. The leaf size is similar to that of HB. The leaves of all leaf belts are of good tissue matter content, with values higher or very close to the control. The average yield/dka is 102,4-133,6% higher than that of cv. HB163. Exceptions are the cytoplasms of (exc) and (afr), which carry genes affecting negatively plant development, with 103,3 cm and 106,8 cm height plus 23 and 25 leaves respectively and decreased yield. All alloplasmic lines except those of (rus) and (ben) are of higher % content of first grade prime matter (11,2%-15,2%) compared with cv. HB163 (9,8%). The highest total income per dka was provided by the alloplasmic lines with cytoplasm from (mar) –135,7%, (vel) –129,6% and (amp) –122,8%. The CMS lines with cytoplasms (vel), (mar), (amp), (lon) and (pan) are identified as most appropriate for further breeding studies.