Multi-omics data revealed NtWRKY75 involved in trichrome development of Nicotiana tabacum
Tobacco leaf is densely covered with various types of trichomes, which play important roles in stress responses and aroma components. In order to detect important regulatory components involved in trichome development, we first tried to identify the variations using resequencing datasets of 254 different cultivars. Then, the potential candidates were identified by genome wide association analysis. The transcriptome level of these candidates was further checked between two cultivars TI1068 and i35 (TI1068 was high-secretory, whereas i35 was low-secretory). Finally, NtWRKY75 was regarded as one of the important potential candidates that may regulate trichome development. RNAi result of NtWRKY75 in K326 indicated that the trichome was much reduced compared with the wild-type. Using the gene regulatory network of tobacco, our study revealed that NtWRKY75 may regulate trichome development by specific LRR receptor-like kinase.