Tobacco smoke constituents have been routinely analysed using either single analyte methods or methods for the determination of a class of substances with very similar physico-chemical properties. To enable their quantitative determination at sometimes very low levels (parts per billion), the analytical approach may comprise multiple sample preparation and clean-up steps in order to remove unwanted matrix artefacts. As well as methods for the quantitative determination of cigarette smoke constituents, for example the FDA list of Harmful and Potentially Harmful Constituents, the availability of screening techniques for multiple classes of constituents is desirable for research purposes and to facilitate the development of quantitative methods, which can be effort intensive. Furthermore, screening techniques have potentially high value as independent or orthogonal methods of measurement confirmation for a wide range of substances of different physico-chemical characteristics. We are evaluating the capability of Nuclear Magnetic Resonance (NMR) spectroscopy and liquid chromatography / high resolution mass spectrometry. Thirty three constituents (anticipated to be compatible with NMR) representing different chemical classes were selected in order to evaluate the potential of NMR. A database of these constituents was created and used for their identification and semi-quantification in mainstream tobacco smoke extracts. Of these 33, 20 were identified and their approximate Limits of Quantification were determined.