TSRC, Tob. Sci. Res. Conf., 2014, 68, abstr. 82

Permeable mechanism of complex enzymes for improving casing absorption in tobacco leaves

QIU Changgui(1); HOU Ying(1); LIU Jihui(2); WANG Hui(2); HUA Yikun(2); WANG Xianguo(2); YANG Shengjiang(3); HUANG Kaixin(3)
(1) Yunnan Reascend Tobacco Technology (Group) Co. Ltd., Kunming, China; (2) Technology Centre of Hongyun Honghe Tobacco (Group) Co., Ltd., Kunming, China; (3) Kunming Weinuo Jinshen Biological Engineering Co., Ltd., Kunming, China

In order to investigate the distribution characteristics and permeable mechanism of casing materials absorbed on the surface of tobacco leaves, the epicuticular waxes on the surface of the tobacco leaf were processed by using methylene chloride and complex enzymes. To compare the impact of casing materials absorption of tobacco leaves in the two different methods by glucose marked using 3-amino-9-ethylcarbazole (AEC) was sprayed onto tobacco leaf and observed with fluorescence microscopy. The results showed that (1) the epicuticular wax content of the tobacco leaf was 5,896 µg/g, (2) initially, glucose molecules penetrated inward from the intercellular space of the epidermal cells in tobacco, and (3) the penetration rate is relatively low in the stomates. This could effectively improve the permeation rate of glucose and promote the casing absorption of the tobacco leaf after degradation of the epicuticular waxes on tobacco leaves.