This paper describes the results of trials in which selected isolate of B. cereus was applied to tobacco seedling roots to determine its effect on the control of black-shank disease in artificially infected soil. The roots of tobacco plants from several varieties with known resistance to Phytophthora parasitica var. nicotianae race O ready for transplanting were treated with bacterial culture and culture filtrate from B. cereus strain by deeping the roots. Treated tobacco plants were transplanted in infected soil with zoospores and mycilium from P. nicotianae. The experiments were conducted in controlled conditions suitable for developing of black-shank disease symptoms. The data were reported as a percentage of the survived treated tobacco plants. The isolate of B. cereus provided significant long protection against tobacco black-shank pathogen. These experiments suggest that the B. cereus strain appears to have a potential as a biocontrol agent for black-shank disease. The next tests will be performed in the field in naturally infected soil.