CORESTA Meeting, Agronomy/Phytopathology, Suzhou, 1999, AP45

Potential of molecular marker analysis for assessment of genetic diversity in tobacco varieties

Philip Morris Europe, R&D, Neuchatel, Switzerland.
The objective of the study was to investigate the genetic diversity of tobacco varieties by developing polymerase chain reaction (PCR)-based procedures that can detect DNA polymorphisms in tobacco. An effective DNA extraction method is required in order to establish genetic profiling in different types of tobacco. Standard methods of extraction, purification and amplification of plant genomic DNA are not effective on tissues of cured tobacco leaf. We describe here a rapid method that is suitable to isolate PCR-quality genomic DNA from cured leaf of flue-cured, oriental and burley tobacco varieties. In the last decade a variety of genetic fingerprinting techniques have been developed and are now frequently used in plant genomic studies. These techniques such as amplified fragment length polymorphism (AFLP) and random amplified polymorphic DNA (RAPD), allow to detect DNA polymorphism for variety identification, marker assisted selection (MAS) and the identification of quantitative trait loci (QTLs). The results of the evaluation of the potential of AFLP and RAPD techniques for the genetic fingerprinting of tobacco varieties will be presented.