The antiviral activities against TMV of crude protein extracts from 18 plants of 13 families were determined by local lesion assay. The results showed that the inhibition effects of different protein extracts on TMV infected Nicotiana glutinosa were varied. The inhibition rates of six extracts were over 50%, among them, those of Spinacia oleracea and Albizzia julibrissin Durazz extracts reached 97.44% and 96.92%, respectively. The crude extracts from Spinacia oleracea and Albizzia julibrissin Durazz were further isolated primarily by ammonium sulfate precipitation, and the inhibition effects of the resulted protein fractions were determined. The results showed that the two protein fractions from Spinacia oleracea extract presented very high antiviral activity; for Albizzia julibrissin Durazz extract, only the fractions precipitated from ammonium sulfate at 40%-80% saturation showed relatively high antiviral activity.