TSRC, Tob. Sci. Res. Conf., 2010, 64, abstr. 12

Quantitation of pesticides in flue-cured tobacco by comprehensive two-dimensional gas chromatography-time of flight mass spectrometry (GCXGC-TOFMS)

BINKLEY J.; PUGH S.
LECO Corporation, Saint Joseph, MI, USA

Flue-curing is a tobacco curing process used to produce cigarette tobacco. The process typically takes approximately a week and generates a tobacco that is high in sugar with a medium to high nicotine content. Tobacco is known to contain thousands of analytes making it an extremely difficult matrix in which to identify pesticide residues. Current methodologies incorporate the use of complex, time consuming sample cleanup techniques to eliminate much of the matrix interference prior to GC-MS analysis. This poster will show how the increased peak capacity of comprehensive two-dimensional gas chromatography provides the ability to effectively separate pesticides from the complex tobacco sample matrix while minimizing the need for extensive sample cleanup methods. In addition, the cryo-focussing effects of thermal modulation increases detectability allowing lower levels of these pesticide residues to be determined. The use of a Time of Flight Mass Spectrometer provides the ability to acquire full mass range spectra without sacrificing sensitivity. This is beneficial for detecting not only target pesticide analytes, but also new and emerging contaminants.Quantitative GCxGC-TOFMS data from two flue-cured tobacco samples will be discussed. Matrix matched GCxGC-TOFMS calibration curves were generated for this work and will also be shown in this poster.