The regulation of microorganism on the formation of TSNAs in tobacco
TSNAs in tobacco are directly approved to be the carcinogens which is getting more and more attention than before. The regulation of TSNAs is becoming one of the most important topics of tobacco harm reduction. It has been shown that TSNAs formation is significantly interacted with the microbes in the flue-curing process. Therefore, the objective of this study is to explore the effects of microbial communities on the formation of tobacco TSNAs and further confirm the relationship between them. Three series of studies were conducted to explore the effects of microbial communities on the TSNAs formation, including fresh leave cultivation, air-curing process, cured leave cultivation. The sequencing method was used to identify the type and function of each strain. This study found that a microorganism (J54) could significantly increase the content of TSNAs in tobacco, while the other (05-5402) could significantly reduce the content of TSNAs to a large extent and other microorganisms were less obvious. Sequencing results show that J54 belongs to Pseudomonas sp., and 05-5402 belongs to Bacillus pumilus. This study clarifies the positive and negative effects of several strains on tobacco TSNAs formation and would play an important role in reducing carcinogens for tobacco. The reduction effect can be directly used in the development of biological method to reduce harmful contents, at the same time, the microorganism (J54) could provide the target for reducing harm study in tobacco.