In order to investigate the relationship between the derivative appearance quality indexes, which have not been involved in the standard of Flue-cured Tobacco (GB2635-1992), but often used in tobacco quality evaluation, and intrinsic traits of flue-cured tobacco, 110 cured tobacco samples were collected from Hunan, Shaanxi, Sichuan, Inner Mongolia, Liaoning and Shandong provinces (autonomous region), and the relationships of the derivative indexes, luster, elasticity and fineness with intrinsic traits were studied. The results showed that: 1) luster and fineness extremely significantly positively correlated with sugar content in tobacco, while extremely significantly negatively correlated with the contents of nicotine and total nitrogen; 2) luster extremely significantly positively correlated with offensive odor, aftertaste and irritation indexes, and significantly positively correlated with total panel test scores; fineness extremely significantly positively correlated with aroma quality, offensive odor, aftertaste, irritation, and total panel test scores; 3) general speaking, elasticity affected intrinsic traits not so strong as luster and fineness, it suggested that luster and fineness could be used as evaluation indexes for appearance quality evaluation.