Report on biological researches using a heated cigarette. Part 4: In vitro oxidative stress assays
Mainstream cigarette smoke (MCS) is a mixture of several thousand constituents and is reported to include many oxidants, such as reactive oxygen species (ROS). ROS induced oxidative damage at molecular and cellular levels. At the 2010 CORESTA Congress (Edinburgh), we presented investigations regarding the cigarette smoke constituent associated with 8-hydroxydeoxyguanosine (8-OHdG) induced in cultured cells and oxidised low-density lipoprotein (ox-LDL) by aqueous extracts of MCS. Our results showed that the gas vapour phase (GVP) was more involved in the induction of both 8-OHdG and ox-LDL than the particulate phase in MCS, and the acrolein in MCS was a major constituent in the induction of 8-OHdG and ox-LDL.
In this study, both oxidative stress assays were applied for estimating the in vitro biological activity of a Heated cigarette (HC) that we developed. The Kentucky reference cigarette, 3R4F, and a commercial cigarette (cigarette C) were used for comparing the activity of a HC. The whole smoke-bubbled PBS (WS-PBS) of a HC indicated less 8-OHdG induction than these combusted cigarettes, while the GVP-PBS of a HC showed the same tendency for 8-OHdG induction. Additionally, both the WS-PBS and GVP-PBS of a HC showed the lowest ox-LDL induction in this experiment.