Search Results

27 results

  1. CORESTA Meeting, Smoke Science/Product Technology, 2017, Kitzbühel, STPOST 45 (also presented at TSRC 2017)

    Stability of the certified 1R6F reference cigarette

    JI H.(1); WU Y.(1); FANNIN F.F.(2); BUSH L.P.(2)
    (1) University of Kentucky, Center of Tobacco Reference Products, Lexington, KY, U.S.A.; (2) University of Kentucky, Department of Plant and Soil Sciences, Lexington, KY, U.S.A.
    University of Kentucky Center of Tobacco Reference Products (CTRP) has provided reference cigarettes for almost 50 years. These reference cigarettes are widely used in tobacco research including analytical method development and modified risk tobacco ...
  2. TSRC, Tob. Sci. Res. Conf., 2017, 71, abstr. 074

    The enantiomeric composition of N'-nitrosonornicotine in air-cured tobacco influenced by nicotine demethylation

    CAI Bin(1); JI Huihua(2); FANNIN F.F.(2); BUSH L.P.(2)
    (1) Guizhou Academy of Tobacco Science, Guiyang City, China; (2) University of Kentucky, Lexington, KY, USA
    N'-nitrosonornicotine (NNN) is a potent and organ-specific carcinogen found in tobacco and tobacco smoke in substantial amounts. The aim of this study was to investigate the variations in NNN enantiomeric composition in nicotine demethylase mutants. ...
  3. CORESTA Meeting, Agronomy/Phytopathology, 2013, Brufa di Torgiano, AP 28

    Changes in TSNA contents during tobacco storage and the effect of temperature and nitrate level on TSNA formation

    SHI Hongzhi(1); WANG Ruiyun(1); BUSH L.P.(2); ZHOU Jun(3); FANNIN F.F.(2); BAI Ruoshi(3); YANG Huijuan(1)
    (1) Henan Agricultural University, National Tobacco Cultivation & Physiology & Biochemistry Research Center, Zhengzhou, P.R. China; (2) University of Kentucky, Lexington, KY, U.S.A.; (3) Beijing Cigarette Factory of Shanghai Tobacco Group, Beijing, P.R. China
    Samples of Burley, sun-cured and flue-cured tobacco from the main producing areas of relevant tobacco types in China were collected to study the changes in tobacco-specific nitrosamine (TSNA) contents during storage and to investigate the effect of ...
  4. 45th TWC, Tob. Work. Conf., 2012, abstr. 42

    Selection for the low alkaloid trait: Part 1 - phenotypic selection

    JACK A.M.; FANNIN F.F.; JI H.; YANG W.; BUSH L.P.
    Kentucky Tobacco Research & Development Center, University of Kentucky, 322 Plant Science Building, Lexington, KY 40546 USA
    The objective of this study was to find the best sampling stage for phenotypic selection of low alkaloid genotypes. Traditionally, breeders sampled mature plants in which the alkaloids were higher and could be measured more easily. However, with modern ...
  5. CORESTA Congress, Sapporo, 2012, Agronomy/Phytopathology Groups, AP 09

    TSNA accumulation in three Burley varieties under three curing regimes

    JACK A.M.; FISHER C.R.; SCHOERGENDORFER A.; JI H.; FANNIN F.F.; BUSH L.P.
    University of Kentucky, Lexington, KY, USA
    Previous work showed that NCBH 129LC had higher accumulation of tobacco specific nitrosamines (TSNAs) than KT 204LC when cured on a wagon covered with plastic; conditions very favorable for TSNA accumulation. However, other researchers found that when the ...
  6. CORESTA Congress, Sapporo, 2012, Smoke Science/Product Technology Groups, SSPTPOST 31

    The use of nicotine to improve the stability of tobacco specific N-nitrosamines (TSNAs) standards

    JI H.(1); FANNIN F.F.(1); JACK A.M.(2); BUSH L.P.(1)
    (1) Department of Plant and Soil Sciences, University of Kentucky, Lexington, KY, USA; (2) Kentucky Tobacco Research and Development Center, University of Kentucky, Lexington, KY, USA
    Analysis of tobacco specific N-nitrosamines (TSNAs) by gas chromatography (GC) using a thermal energy analyzer (TEA) detector has been the preferred method until quite recently. Although liquid chromatogram/mass spectrometer/mass spectrometer (LC/MS/MS) ...
  7. CORESTA Meeting, Agronomy/Phytopathology, 2011, Santiago, AP 04

    TSNA accumulation in two burley tobacco cultivars

    JACK A.M.(1); FISHER C.R.(1); SCHOERGENDORFER A.(1); FANNIN F.F.(1); DANEHOWER D.(2); BUSH L.P.(1)
    (1) University of Kentucky, Lexington, KY, USA; (2) North Carolina State University, Raleigh, NC, USA
    Differences in TSNA accumulation between burley genotypes can often be attributed to differences in nicotine conversion and/or differences in amount of total alkaloids. However, several researchers have observed differences in TSNAs between certain ...
  8. CORESTA Congress, Edinburgh, 2010, AP 01; TSRC, Tob. Sci. Res. Conf., 2010, 64, abstr. 45

    Nicotine is enantioselectively demethylated by cytochrome P450 enzyme CYP82E4

    CAI B.; FANNIN F.F.; CHAPPELL J.; BUSH L.P.
    University of Kentucky, Dept. of Plant & Soil Sciences, Lexington, KY, USA
    Nicotine and nornicotine are two of the dominant alkaloids found in tobacco (N. tabacum L.), and known precursors to 4-(methylnitrosamino)-1-(3-pyridyl)-1-butanone (NNK) and N'-nitrosonornicotine (NNN).  In tobacco nicotine is N-demethylated to ...