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5 results

  1. Tobacco Science & Technology, 2011, 10, p. 65-69., ISSN.1002-0861

    Development and preliminary application of one-step RT-PCR kit for detection of tobacco CMV

    ZHANG JUN-QI(1); LIU XIANG-BING(2); LUO ZHAO-PENG(1); YANG JUN(1); SONG JI-ZHEN(1); YIN QI-SHENG(1); SONG HAO(2)
    1. Zhengzhou Tobacco Research Institute of CNTC, Zhengzhou 450001, P.R. China; 2. China Tobacco Hunan Industrial Co., Ltd., Changsha 410007, P.R. China
    To detect tobacco CMV quickly and accurately, a pair of specific primers was designed according to the relatively conservative region in the CMV genomic sequence, and enzymes and buffers needed for reverse transcription and PCR were integrated. By ...
  2. CORESTA Congress, Edinburgh, 2010, AP 07

    Isolation of protein from Spinacia oleracea against tobacco mosaic virus and research on the antiviral mechanism

    JIN Guanghui; YANG Jun; YIN Qisheng; SONG Jizhen; JIN Lifeng; XUE Chaoqun; LUO Chaopeng
    Zhengzhou Tobacco Research Institute of CNTC, Zhengzhou, Henan, China
    Tobacco mosaic virus (TMV) is a worldwide-occurring tobacco viral disease and usually results in big losses in tobacco production. How to inhibit TMV infection and replication has become a hotspot in the field of plant virus disease control. Antiviral ...
  3. Tobacco Science & Technology, 2010, 6, p. 73-76., ISSN.1002-0861

    Preliminary screening of bioactive substances from plants against tobacco mosaic virus

    JIN GUANG-HUI; YIN QI-SHENG; YANG JUN; LI FENG; JIN LI-FENG; LUO ZHAO-PENG; WANG XIN-MIN
    Zhengzhou Tobacco Research Institute of CNTC, Zhengzhou 450001, P.R. China
    The antiviral activities against TMV of crude protein extracts from 18 plants of 13 families were determined by local lesion assay. The results showed that the inhibition effects of different protein extracts on TMV infected Nicotiana glutinosa were ...
  4. CORESTA Congress, Shanghai, 2008, AP 13

    Insect-proof plastic bag (inside lining) can prevent tobacco beetles from second invasion

    SONG Jizhen; ZHOU Hanping; XI Jiaqin; YIN Qisheng; YANG Jun; XIE Jianping
    Zhengzhou Tobacco Research Institute of CNTC, Zhengzhou, Henan, China
    The protective agent A, the mixture C and the mixture D were screened from five kinds of protective agent, and they have good touch-killing effect on tobacco beetles. Six kinds of insect-proof plastic bags were made that were marked A1, A2, C1, C2, D1, D2 ...
  5. Tobacco Science & Technology, 2007, 12, p. 46-50, ISSN.1002-0861.

    Validity of advance nicotine conversion induced by 2-chloroethyl phosphonic acid in Burley

    CAI BIN; YIN QI-SHENG; YANG JUN
    Zhengzhou Tobacco Research Institute of CNTC, Zhengzhou, Henan, China
    In order to evaluate the validity of early nicotine conversion induced by 2-chloroethyl phosphonic acid in Burley tobacco, the nicotine conversion in 3 Burley cultivars and 1 improved cultivar from Hubei and Hainan treated with 2-chloroethyl phosphonic ...