Search Results

21 results

  1. CORESTA Congress, Kunming, 2018, Smoke Science/Product Technology Groups, STPOST 52

    Biological test procedure for fresh generated cigarette smoke and e-cigarette aerosols under air-liquid interface (ALI) exposure in 24 and 96 multi-well plates

    WIECZOREK R.(1); TRELLES STICKEN E.(1); WALELE T.(2); CZEKALA L.(3); SIMMS L.(3)
    (1) Reemtsma Cigarettenfabriken GmbH, Imperial Brands plc, Hamburg, Germany; (2) Fontem Ventures, Amsterdam, The Netherlands; (3) Imperial Tobacco Limited, Bristol, U.K.
    Cigarette smoke and e cigarette aerosol contain particulate matter/liquid droplets in the solid phase as well as volatile substances in the gas phase. Both fractions are of potential toxicological relevance and should be considered when assessing ...
  2. CORESTA Congress, Kunming, 2018, Smoke Science/Product Technology Groups, STPOST 53

    Use of human derived cell lines to increase the biological relevance of cigarette condensate, non-tobacco materials and e-liquids testing

    WIECZOREK R.(1); TRELLES STICKEN E.(1); WALELE T.(2); CZEKALA L.(3); SIMMS L.(3)
    (1) Reemtsma Cigarettenfabriken GmbH, Imperial Brands plc, Hamburg, Germany; (2) Fontem Ventures, Amsterdam, The Netherlands; (3) Imperial Tobacco Limited, Bristol, U.K.
    The in vitro testing strategy of tobacco products is mainly based on the testing of extracts from cigarette smoke condensates. An accepted industry wide assay battery has been developed over recent years which delivers reliable results for the ...
  3. CORESTA Meeting, Smoke Science/Product Technology, 2017, Kitzbühel, STPOST 22

    Dosimetry: TPM and nicotine delivery of combusted tobacco products to the 24 and 96 Multi Well Plate and inserts on Smoke Aerosol Exposure In Vitro System (SAEIVS)

    WIECZOREK R.(1); TRELLES STICKEN E.(1); BODE L.M.(1); SIMMS L.(2)
    (1) Reemtsma Cigarettenfabriken GmbH (an Imperial Brands PLC Company), Hamburg, Germany; (2) Imperial Tobacco Limited, Bristol, U.K.
    In vitro exposure systems are key tools for the risk assessment of emerging electronic nicotine delivery systems as well as for conventional tobacco products. The Smoke Aerosol Exposure in vitro System (SAEIVS) used at Imperial Tobacco Limited is designed ...
  4. CORESTA Meeting, Smoke Science/Product Technology, 2017, Kitzbühel, STPOST 23

    Dosimetry: the effects of cigarette smoke dilution on nicotine delivery in a 24 and 96 well format using Smoke Aerosol Exposure In Vitro System (SAEIVS)

    BODE L.M.(1); ROEWER K.(1); OTTE S.(1); WIECZOREK R.(1); SIMMS L.(2)
    (1) Reemtsma Cigarettenfabriken GmbH (an Imperial Brands PLC Company), Hamburg, Germany; (2) Imperial Tobacco Limited, Bristol, U.K.
    In vitro aerosol exposure systems are useful tools for screening of potential toxic effects of freshly generated smoke of conventional cigarettes [1]. An in house platform “Smoke Aerosol Exposure In Vitro System (SAEIVS)” (assembled by Burghart ...
  5. CORESTA Congress, Berlin, 2016, Smoke Science/Product Technology Groups, STPOST 26

    A review of aerosol exposure systems relative to the analysis of cytotoxicity: a CORESTA In vitro Toxicity Testing Sub-Group perspective

    THORNE D.(1); WIECZOREK R.(2); FUKUSHIMA T.(3); SHIN H.J.(4); LEVERETTE R.(5); BALLANTYNE M.(6); LI X.(7); BOMBICK B.(5)
    (1) British American Tobacco, R&D Centre, Southampton, U.K.; (2) Reemtsma Cigarettenfabriken GmbH (an Imperial Brands PLC Company), Hamburg, Germany; (3) Japan Tobacco Inc., Scientific Product Assessment Centre, Yokohama, Kanagawa, Japan; (4) KT&G Corporation, Daejeon, South Korea; (5) RAI Services Company, Winston-Salem, NC, U.S.A.; (6) Covance Laboratories Ltd, Harrogate, U.K.; (7) Zhengzhou Tobacco Research Institute of CNTC, Zhengzhou, P.R. China
    Aerosol exposure systems offer researchers a variety of ways to customise the exposure set-up, modify experimental parameters and provide a novel and versatile platform for in vitro aerosol research. These systems produce an aerosol that more closely ...
  6. CORESTA Congress, Berlin, 2016, Smoke Science/Product Technology Groups, STPOST 32

    Cellular transformation activity of different tobacco condensates in the Bhas 42 cell assay

    STEVENSON M.(1); WIECZOREK R.(2); SIMMS L.(1); PANT K.(3)
    (1) Imperial Tobacco Limited, Bristol, U.K.; (2) Reemtsma Cigarettenfabriken GmbH (an Imperial Brands PLC Company), Hamburg, Germany; (3) BioReliance, Sigma-Aldrich Corp., Rockville, MD, U.S.A.
    Carcinogenesis has been described as a multi-stage process comprising of initiation, promotion and progression. Smoking is a cause of serious disease in smokers, including lung cancer. Determining the carcinogenic potential of a tobacco product ingredient ...
  7. CORESTA Meeting, Smoke Science/Product Technology, 2015, Jeju, ST 44

    Optimisation of in vitro standard testing of e-vapour and heated tobacco products

    WIECZOREK R.; TRELLES STICKEN E.
    Imperial Tobacco Group, Reemtsma Cigarettenfabriken GmbH, Biological Labs Hamburg, Hamburg, Germany
    The risk assessment of tobacco products includes a number of biological in vitro test assays which have become de facto standards over the last decades. These test methods are designed to assess specific toxicological end points and are able to ...
  8. CORESTA Meeting, Smoke Science/Product Technology, 2015, Jeju, STPOST 14

    Use of the TK6 human lymphoblastoid cell line for the assessment of the genotoxic potential of complex mixtures

    TRELLES STICKEN E.; WIECZOREK R.
    Imperial Tobacco Group, Reemtsma Cigarettenfabriken GmbH, Biological Labs Hamburg, Hamburg, Germany
    There has been a significant focus over recent years to evaluate the relevance of in vitro micronucleus (IVM) data obtained with cell lines of different origin and different status regarding the p53 checkpoint. Those evaluations and additional ...
  9. CORESTA Congress, Quebec, 2014, Smoke Science/Product Technology Groups, ST 80

    The CORESTA recommended in vitro test battery alongside the National Research Council vision for toxicity testing in the 21st Century

    SIMMS L.(1); WIECZOREK R.(2); TRELLES STICKEN E.(2)
    (1) Imperial Tobacco Ltd, Bristol, U.K.; (2) Imperial Tobacco Group, Reemtsma Cigarettenfabriken GmbH, Biological Labs Hamburg, Hamburg, Germany
    The CORESTA In vitro Toxicity Testing of Tobacco Smoke Task Force recommended a test battery in 2004 which has been subsequently used by the tobacco industry. A similar in vitro test battery has been used by the International Community on Harmonisation ...
  10. CORESTA Congress, Quebec, 2014, Smoke Science/Product Technology Groups, STPOST 25

    Increasing the Salmonella typhimurium reverse mutation assay’s sensitivity following exposure to fresh aerosols

    TRELLES STICKEN E.; WIECZOREK R.
    Imperial Tobacco Group, Reemtsma Cigarettenfabriken GmbH, Biological Labs Hamburg, Hamburg, Germany
    For the in vitro toxicological assessment of current and next generation products, including e-vapour products, the standard in vitro test battery developed for conventional combustible products needs further refinement in terms of sensitivity to ensure ...