1. CORESTA Congress, Kunming, 2018, Agronomy/Phytopathology Groups, APPOST 13

    Genetic diversity of potato virus Y infecting tobacco in major tobacco growing fields in Northern Vietnam

    NGUYEN VAN C.(1); NGUYEN VAN D.(2); VAN LE S.(2)
    (1) Vietnam Tobacco Institute, Hanoi, Vietnam; (2) Vietnam Institute of Biotechnology, Hanoi, Vietnam
    Potato virus Y (PVY) is the type member of genus Potyvirus and one of the most common species and most pernicious of Potyvirus. PVY has a wide host range and infects several important solanaceous crops, including potato, tomato and tobacco. In Vietnam, ...
  2. CORESTA Congress, Kunming, 2018, Agronomy/Phytopathology Groups, APPOST 15

    Effect of potato virus Y on growth, yield and chemical composition of flue-cured tobacco in Northern Vietnam

    NGUYEN VAN C.; HONG NGUYEN T.; THI DO T.
    Vietnam Tobacco Institute, Hanoi, Vietnam
    Potato virus Y (PVY) is a plant virus that causes significant losses to tobacco. PVY damage is severe in the tobacco growing provinces Lang Son and Bac Giang with disease rates from 10-45 %, with some areas infected 100 %. The objective of this study was ...
  3. CORESTA Congress, Kunming, 2018, Smoke Science/Product Technology Groups, ST 25

    Misperception of e-cigarette harm growing among American adults, 2013-2015

    VERRON T.(1); GUO M.(1); VARIGNON B.(1); O'CONNELL G.(2); WALELE T.(2); CAHOURS X.(1)
    (1) SEITA - Imperial Brands plc, Paris, France; (2) Fontem Ventures B.V., Amsterdam, The Netherlands
    Electronic cigarettes (e cigarettes) have been characterised as significantly less harmful than smoked tobacco by an increasing number of public health authorities. However, the proportion of American adults who perceive e cigarettes to be equally or more ...
  4. CORESTA Congress, Kunming, 2018, Smoke Science/Product Technology Groups, STPOST 39

    The use of high content screening (HCS) in human primary lung cells to assess e-liquids of differing flavors and nicotine concentrations

    SIMMS L.(1); CZEKALA L.(1); STEVENSON M.(1); PHILLIPS G.(1); WALELE T.(2)
    (1) Imperial Brands, Bristol, U.K.; (2) Fontem Ventures, Amsterdam, The Netherlands
    Due to the current scientific debate and evolving regulatory landscape, new assays are needed to quickly determine the subtle biological response of e-liquids for both stewardship and product development purposes. For stewardship, all ingredients in the e ...
  5. CORESTA Congress, Kunming, 2018, Smoke Science/Product Technology Groups, STPOST 52

    Biological test procedure for fresh generated cigarette smoke and e-cigarette aerosols under air-liquid interface (ALI) exposure in 24 and 96 multi-well plates

    WIECZOREK R.(1); TRELLES STICKEN E.(1); WALELE T.(2); CZEKALA L.(3); SIMMS L.(3)
    (1) Reemtsma Cigarettenfabriken GmbH, Imperial Brands plc, Hamburg, Germany; (2) Fontem Ventures, Amsterdam, The Netherlands; (3) Imperial Tobacco Limited, Bristol, U.K.
    Cigarette smoke and e cigarette aerosol contain particulate matter/liquid droplets in the solid phase as well as volatile substances in the gas phase. Both fractions are of potential toxicological relevance and should be considered when assessing ...
  6. CORESTA Congress, Kunming, 2018, Smoke Science/Product Technology Groups, STPOST 53

    Use of human derived cell lines to increase the biological relevance of cigarette condensate, non-tobacco materials and e-liquids testing

    WIECZOREK R.(1); TRELLES STICKEN E.(1); WALELE T.(2); CZEKALA L.(3); SIMMS L.(3)
    (1) Reemtsma Cigarettenfabriken GmbH, Imperial Brands plc, Hamburg, Germany; (2) Fontem Ventures, Amsterdam, The Netherlands; (3) Imperial Tobacco Limited, Bristol, U.K.
    The in vitro testing strategy of tobacco products is mainly based on the testing of extracts from cigarette smoke condensates. An accepted industry wide assay battery has been developed over recent years which delivers reliable results for the ...
  7. TSRC, Tob. Sci. Res. Conf., 2018, 72, abstr. 044

    Comparative measurements of puff aerosol size and concentration of combustion, heated tobacco, and liquid e-cigarettes by light scattering method

    HOLVE D.J.(1); HOORNSTRA J.(2)
    (1) Envirometrix Instruments LLC, Berkeley, CA, USA; (2) Envirometrix Instruments LLC, Sint Maartensbrug, Netherlands
    In a previous presentation at the 2015 TSRC, the development and application of a new light scattering instrument based on Mie theory (Orion-A) was presented (ref 1). Further developments and improvements have been implemented on the Orion instrument, ...
  8. CORESTA Meeting, Smoke Science/Product Technology, 2017, Kitzbühel, ST 22

    Estimation of e-cigarette aerosol yields based on puff duration

    JULIEN R.(1); TSCHIERSKE N.(2); VARIGNON B.(1); TROUDE V.(1); DESTRUHAUT S.(1); WALELE T.(3); COLARD S.(1); CAHOURS X.(1)
    (1) SEITA-Imperial Tobacco Limited, Fleury-les-Aubrais, France; (2) Reemtsma Cigarettenfabriken GmbH (an Imperial Brands PLC Company), Hamburg, Germany; (3) Fontem Ventures B.V. (an Imperial Brands PLC Company), Amsterdam, The Netherlands
    There are regulatory requirements in quantifying and comparing the emission levels of major and minor aerosol constituents from e-cigarettes. Up until the recent publication in 2015 of CORESTA Recommended Method (CRM) No. 81- " Routine Analytical ...
  9. CORESTA Meeting, Smoke Science/Product Technology, 2017, Kitzbühel, ST 62

    Overview of in vitro methods used to assess e-cigarettes based on “Toxicity Testing in 21st Century” principles

    SIMMS L.(1); STEVENSON M.(1); CZEKALA L.(1); TSCHIERSKE N.(1); WALELE T.(2)
    (1) Imperial Tobacco Ltd, Bristol, U.K.; (2) Fontem Ventures B.V. (an Imperial Brands PLC Company), Amsterdam, The Netherlands
    When the National Academies of Sciences released “Toxicity Testing in the 21st Century: A Vision and a Strategy” a new toxicological paradigm was created, focusing on the use of human cell lines and the disruption of key cellular pathways. In keeping with ...
  10. CORESTA Meeting, Smoke Science/Product Technology, 2017, Kitzbühel, ST 63 (also presented at TSRC 2017)

    A computational model to characterize the Vitrocell® Cell Exposure System for evaluation of aerosols

    CASTRO N.(1); ROSTAMI A.(1); KUCZAJ A.(2,3); LUCCI F.(2); OLDHAM M.J.(1); PITHAWALLA Y.B.(1)
    (1) Altria Client Services LLC, Center for Research and Technology, Richmond, VA, U.S.A.; (2) Philip Morris Products S.A. (part of Philip Morris International group of companies), PMI R&D, Neuchâtel, Switzerland; (3) Multiscale Modeling and Simulation, Faculty EEMCS, University of Twente, Enschede, The Netherlands
    In vitro exposure systems can be used as tools for toxicological assessment of e-cigarette aerosols. The VITROCELL ® exposure system is designed such that exposure of cell cultures to the aerosol occurs at the air/liquid interface, which is relevant to e ...