1. CORESTA Congress, Kunming, 2018, Smoke Science/Product Technology Groups, STPOST 52

    Biological test procedure for fresh generated cigarette smoke and e-cigarette aerosols under air-liquid interface (ALI) exposure in 24 and 96 multi-well plates

    WIECZOREK R.(1); TRELLES STICKEN E.(1); WALELE T.(2); CZEKALA L.(3); SIMMS L.(3)
    (1) Reemtsma Cigarettenfabriken GmbH, Imperial Brands plc, Hamburg, Germany; (2) Fontem Ventures, Amsterdam, The Netherlands; (3) Imperial Tobacco Limited, Bristol, U.K.
    Cigarette smoke and e cigarette aerosol contain particulate matter/liquid droplets in the solid phase as well as volatile substances in the gas phase. Both fractions are of potential toxicological relevance and should be considered when assessing...
  2. CORESTA Congress, Kunming, 2018, Smoke Science/Product Technology Groups, STPOST 53

    Use of human derived cell lines to increase the biological relevance of cigarette condensate, non-tobacco materials and e-liquids testing

    WIECZOREK R.(1); TRELLES STICKEN E.(1); WALELE T.(2); CZEKALA L.(3); SIMMS L.(3)
    (1) Reemtsma Cigarettenfabriken GmbH, Imperial Brands plc, Hamburg, Germany; (2) Fontem Ventures, Amsterdam, The Netherlands; (3) Imperial Tobacco Limited, Bristol, U.K.
    The in vitro testing strategy of tobacco products is mainly based on the testing of extracts from cigarette smoke condensates. An accepted industry wide assay battery has been developed over recent years which delivers reliable results for the...
  3. CORESTA Meeting, Smoke Science/Product Technology, 2017, Kitzbühel, STPOST 22

    Dosimetry: TPM and nicotine delivery of combusted tobacco products to the 24 and 96 Multi Well Plate and inserts on Smoke Aerosol Exposure In Vitro System (SAEIVS)

    WIECZOREK R.(1); TRELLES STICKEN E.(1); BODE L.M.(1); SIMMS L.(2)
    (1) Reemtsma Cigarettenfabriken GmbH (an Imperial Brands PLC Company), Hamburg, Germany; (2) Imperial Tobacco Limited, Bristol, U.K.
    In vitro exposure systems are key tools for the risk assessment of emerging electronic nicotine delivery systems as well as for conventional tobacco products. The Smoke Aerosol Exposure in vitro System (SAEIVS) used at Imperial Tobacco Limited is...
  4. CORESTA Meeting, Smoke Science/Product Technology, 2017, Kitzbühel, STPOST 23

    Dosimetry: the effects of cigarette smoke dilution on nicotine delivery in a 24 and 96 well format using Smoke Aerosol Exposure In Vitro System (SAEIVS)

    BODE L.M.(1); ROEWER K.(1); OTTE S.(1); WIECZOREK R.(1); SIMMS L.(2)
    (1) Reemtsma Cigarettenfabriken GmbH (an Imperial Brands PLC Company), Hamburg, Germany; (2) Imperial Tobacco Limited, Bristol, U.K.
    In vitro aerosol exposure systems are useful tools for screening of potential toxic effects of freshly generated smoke of conventional cigarettes[1]. An in house platform “Smoke Aerosol Exposure In Vitro System (SAEIVS)” (assembled by Burghart...
  5. CORESTA Meeting, Smoke Science/Product Technology, 2013, Seville, STPOST 03

    Analytical method to model human mouth-level transfer of ingredients from Swedish pouched snus

    GRAPTON S. (1); GADOIS-POMMEREUL S.(1); TROUDE V.(1); BLANCHET M. (1); CLARKE A.(2); SIMMS L.(2)
    (1) SEITA, Imperial Tobacco Group, Fleury-les-Aubrais, France; (2) Imperial Tobacco Limited, Bristol, U.K.
    In parallel with the human study[1] a laboratory in vitro assay was conducted with three media: artificial saliva, water and 1% ß-cyclodextrin in water, using two snus products that were indistinguishable apart from the flavour application rate. To...