Search Results

26 results

  1. CORESTA Meeting, Agronomy/Phytopathology, 2013, Brufa di Torgiano, AP 15

    Field control efficacy of bio-organic fertiliser fermented with antagonists to tobacco bacterial wilt and its biocontrol mechanisms

    LIU Yanxia; LI Xiang; CAI Liuti; CAO Yi; SHI Junxiong
    Guizhou Tobacco Institute, Jinyang District, Guiyang, P.R. China
    An organic fertiliser made of rapeseed meal and dairy manure with proportion of 1:1 (w:w) was then solid fermented by two antagonists, making it bio-organic fertilizer (BOF). Field experiments were conducted in Anhui Province for two years to investigate ...
  2. CORESTA Congress, Sapporo, 2012, Agronomy/Phytopathology Groups, APPOST 09

    Cloning and bioinformatic analysis of orange protein (OR) gene from Nicotiana tabacum

    LEI Bo(1); LU Kun(2); DING Fu-zhang(1); LIU Xiao-rong(2); HU Zhong-yi(1); PAN Wen-jie(1)
    (1) Guizhou Tobacco Science Research Institute, Guiyang, Guizhou, P.R. China; (2) College of Agronomy and Biothehnology of Southwest University, Chongqing, P.R. China
    Previous microarray results indicated that the orange protein (OR) gene in the carotenoid biosynthesis pathway showed significant differential expression in leaves between moderate and light aroma tobacco areas. In order to obtain the full-length sequence ...
  3. Tobacco Science & Technology, 2012, 1, p. 74-77., ISSN.1002-0861

    Effects of girdling on physical characters and smoking quality of flue-cured tobacco leaves

    YANG Chunyuan
    Guizhou Tobacco Science Research Institute/China Tobacco Southwest Agricultural Experiment Station, Guiyang 550081, P.R. China
    In order to improve cultivation practices and promote leaf quality, the stalk of tobacco plant was girdled after topping. The results showed that the girdling affected physical characters and smoking quality of flue-cured tobacco leaves greatly. The ...
  4. Tobacco Science & Technology, 2012, 10, p. 31-35, ISSN.1002-0861

    Comparison of main secondary metabolite contents in flue-cured tobacco leaves from different areas in Guizhou

    GENG Zhaoliang(1); FENG Yonggang(1); XIANG Zhangmin(1); ZHANG Jie(1); CAI Kai(1); ZHU Xianling(*2)
    (1) Guizhou Tobacco Research Institute, Guiyang 550081, P.R. China; (2) Research Center of Tobacco and Health, University of Science and Technology of China, Hefei 230051, P.R. China
    In order to compare the secondary metabolite contents in flue-cured leaves from different areas in Guizhou Province, the leaf samples of C3F and B2F grades of K326, Yunyan 85, Yunyan 87, Guiyan 1 and Guiyan 201 varieties were collected from Daozhen, ...
  5. Tobacco Science & Technology, 2012, 11, p. 74-78, ISSN.1002-0861

    Influences of arbuscular mycorrhizal fungi on phosphorus content in tobacco planting soil and nutrition absorption of flue-cured tobacco plants

    YAO Juan(1); WANG Maosheng(2); WANG Feng(*1,3); XU Dongqing(2)
    (1) Agricultural College of Guizhou University, Guiyang 550025, P.R. China; (2) Guizhou Tobacco Science Institute, Guiyang 550081, P.R. China; (3) Qianxinan Prefecture Tobacco Company of Guizhou Province, Xingyi 562400, Guizhou, P.R. China
    In order to study the mycorrhizal effects of mycorrhizal seedling after transplanting, three nitrogen (N) levels, 45, 67.5 and 90 kg/ha, were applied in field experiments and the effects of mycorrhizal seedlings on phosphorus (P) content in soil and N and ...
  6. Tobacco Science & Technology, 2012, 3, p. 34-37., ISSN.1002-0861

    Cloning and sequence analysis of T-zds1 gene from flue-cured tobacco

    HU Zhongyi(1) ; LEI Bo(1) ; LIU Dong(2) ; CAI Liuti(1)
    (1) Guizhou Tobacco Research Institute, Guiyang 550081, P.R. China; (2) College of Agriculture, Yangtze University, Jingzhou 434023, Hubei, P.R. China
    Homology cloning and in silico cloning method were used to clone the full length cDNA sequence of tobacco zeta carotene dehydrogenase gene (named T-zds 1) from flue-cured tobacco cultivar, Jiucaiping2, and the primary and secondary structures of its ...
  7. Tobacco Science & Technology, 2012, 4, p. 60-66, ISSN.1002-0861

    Cloning and bioinformatics analysis of orange protein (OR) gene from Nicotiana tabacum

    LEI Bo(1); LU Kun(2); DING Fuzhang(1); LIU Xiaorong(2); HU Zhongyi(1); PAN Wenjie(1)
    (1) Guizhou Tobacco Research Institute, Guiyang 550081, P.R. China; (2) College of Agronomy and Biotechnology of Southwest University, Chongqing 400715, P.R. China
    In order to obtain the full-length sequence of NtOR and reveal its biological function, the full-length cDNA and genomic DNA sequences of NtOR were cloned from cv. K326 using homologous cloning method, and logged in GenBank (accession numbers JN379458 and ...
  8. Tobacco Science & Technology, 2012, 8, p. 54-59, 65, ISSN.1002-0861

    Correlation analysis between nicotine content and medium and trace element contents in flue-cured tobacco leaves from different stalk positions

    CHEN Wei(1); TANG Yuanju(1); PAN Wenjie(1); LI Zhiyong(*2)
    (1) Guizhou Tobacco Research Institute, Guiyang 550081, P.R. China; (2) Guizhou Provincial Tobacco Company, Guiyang 550003, P.R. China
    The relationships between nicotine content and the contents of medium and trace elements in flue-cured tobacco leaves from different stalk positions were analyzed with descriptive statistics analysis, stepwise regression analysis and path analysis. The ...
  9. Tobacco Science & Technology, 2012, 8, p. 70-74, ISSN.1002-0861

    Cloning and sequence analysis of β-carotene hydroxylase gene in Nicotiana tabacum

    LIN Shifeng; WANG Rengang; Wang Yi; REN Xueliang*
    Guizhou Tobacco Research Institute, Guiyang 550081, P.R. China
    In order to effectively modulate the anabolism of carotenoid in tobacco, a β-carotene hydroxylase (BCH) gene related to plant carotenoid anabolism was cloned from Nicotiana tabacum. A cDNA sequence of encoded BCH gene was isolated from tobacco cv. K326 by ...