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6 results

  1. TSRC, Tob. Sci. Res. Conf., 2015, 69, abstr. 101

    Quantitative proteomics analysis the tobacco trichomes under cadmium stress

    FU Qiang; LIN Shifeng; ZOU Jie; YU Jing; ZHANG Xiaolian; REN Xueliang
    Guizhou Academy of Tobacco Sciences, Guiyang City, Guizhou, P.R. China
    Nicotiana tabacum (tobacco) is known to both accumulate and tolerate high levels of heavy metals from polluted soils, its trichomes appeared to be the excretion of toxic metals in the form of inorganic particles To gain a comprehensive understanding of ...
  2. TSRC, Tob. Sci. Res. Conf., 2014, 68, abstr. 30

    Application of TILLING technology to screen for mutations in the tobacco glycosyltransferase gene NtGt1a

    FU Qiang; ZOU Jie; ZHANG Jishun; LIN Shifeng; YU Jing; GUO Yushuang; ZHANG Xiaolian; YU Shizhou; ZHAO Jiehong; REN Xueliang
    Guizhou Academy of Tobacco Science, Guiyang, Guizhou, China
    Targeting Induced Local Lesions IN Genomes (TILLING) combines chemical mutagenesis with high throughput screening to allow for the generation and detection of mutant alleles in selected genes. In this study, TILLING has been applied to produce a series of ...
  3. TSRC, Tob. Sci. Res. Conf., 2014, 68, abstr. 90

    Isolation and functional analysis of an ltr-retrotransposon from tobacco (Nicotiana tabacum)

    GUO Yushuang; LIN Shifeng; YU Jing; ZOU Jie; FU Qiang; YU Shizhou; ZHANG Xiaolian; ZHAO Jiehong; REN Xueliang
    Key Laboratory of Molecular Genetics, Guizhou Academy of Tobacco Sciences, Guiyang, China
    Long terminal repeat (LTR)-retrotransposons are genetic elements that can move and sometimes spread within genomes, and are important for plant genomic organization. In this research, a novel LTR-retrotransposon gene named Ntrt1 was isolated from ...
  4. TSRC, Tob. Sci. Res. Conf., 2013, 67, abstr. 21

    Cloning and characterization of the cysteine proteinase inhibitor (CPI) gene family in tobacco (Nicotiana tabacum L.).

    LIN Shifeng; WANG Rengang; ZOU Jie; FU Qiang; ZHAO Jiehong; REN Xueliang
    Guizhou Academy of Tobacco Science, Guiyang City, P.R. China
    In plants, cysteine proteinase inhibitors (CPI or cystatin) are implicated in biotic and/or abiotic stress responses and developmental regulation. Using the techniques of RT-PCR and SMART RACE, full-length cDNAs of four CPI genes (NtCPI1, NtCPI2, NtCPI3, ...
  5. TSRC, Tob. Sci. Res. Conf., 2013, 67, abstr. 29

    The establishment of the tilling experimental techniques based on capillary electrophoresis.

    FU Qiang; ZOU Jie; ZHANG Jishun; LIN Shifeng; WANG Yi; REN Xueliang
    Guizhou Academy of Tobacco Science, Guiyang City, P.R. China
    TILLING (Targeting Induced Local Lesions in Genomes) is a general reverse-genetic strategy that provides an allelic series of induced point mutations in genes of interest. The CEL I enzyme that specifically cleaves the mismatch in DNA double strands is an ...