Search Results

36 results

  1. CORESTA Congress, Berlin, 2016, Agronomy/Phytopathology Groups, AP 48

    TALEN-mediated mutagenesis of NteIF4E1a leads to potato virus Y (PVY) resistance in flue-cured tobacco (Nicotiana tabacum cv. K326)

    YANG Dahai(1,2,3); YAO Heng(1,2,3); XIE He(1,2,3); BAI Ge(1,2,3); CHEN Xuejun(1,2,3); LI Yongping(1,2,3)
    (1) Tobacco Breeding and Biotechnology Research Center, Yunnan Academy of Tobacco Agricultural Sciences, Kunming, P.R. China; (2) Key Laboratory of Tobacco Biotechnological Breeding, Kunming, P.R. China; (3) National Tobacco Genetic Engineering Research Center, Kunming, P.R. China
    Potato virus Y (PVY) infection causes severe yield reduction and quality decline of cultivated tobacco (Nicotiana tabacum) leaves, and results in large economic losses. In tobacco production, effective control of PVY is urgently needed. Breeding PVY ...
  2. CORESTA Congress, Berlin, 2016, Agronomy/Phytopathology Groups, AP 49

    Construction and genetic evaluation of chromosome segment substitution lines in tobacco (Nicotiana tabacum L.)

    TONG Zhijun; JIAO Fangchan; CHEN Xuejun; XIAO Bingguang
    Yunnan Academy of Tobacco Agricultural Sciences of CNTC, Key Laboratory of Tobacco Biotechnological Breeding, National Tobacco Genetic Engineering Research Center, Kunming, P.R. China
    A set of chromosome segment substitution lines (CSSLs) of tobacco (Nicotiana tabacum L.) was first developed by molecular marker assisted selection (MAS) and successive backcrossing with Y3, the flue-cured tobacco germplasm with comprehensive traits as ...
  3. CORESTA Congress, Berlin, 2016, Smoke Science/Product Technology Groups, STPOST 11

    Quantitative discrimination of the flavour type of flue-cured tobacco and correlation between flavour type and planting area

    LI Chao(1); MIAO Mingming(1); QIN Yunhua(1); LI Exian(2); WU Yiqin(1); XU Yong(1); YANG Guangyu(1); ZHANG Chengming(1)
    (1) Cigarette Product Quality Inspection Institute, Technology Center, China Tobacco Yunnan Industrial Co., Ltd., Kunming, P.R. China; (2) Institute of Biotechnology and Genetic Resources, Yunnan Academy of Agricultural Sciences, Kunming, P.R. China
    To study the relationship between chemical composition and flavour type of flue-cured tobacco, 500 tobacco samples were collected from 29 areas in 14 Chinese provinces in 2012. A classical sampling method was applied. Following relevant methods described ...
  4. CORESTA Meeting, Agronomy/Phytopathology, 2013, Brufa di Torgiano, AP 15

    Field control efficacy of bio-organic fertiliser fermented with antagonists to tobacco bacterial wilt and its biocontrol mechanisms

    LIU Yanxia; LI Xiang; CAI Liuti; CAO Yi; SHI Junxiong
    Guizhou Tobacco Institute, Jinyang District, Guiyang, P.R. China
    An organic fertiliser made of rapeseed meal and dairy manure with proportion of 1:1 (w:w) was then solid fermented by two antagonists, making it bio-organic fertilizer (BOF). Field experiments were conducted in Anhui Province for two years to investigate ...
  5. CORESTA Congress, Sapporo, 2012, Agronomy/Phytopathology Groups, APPOST 09

    Cloning and bioinformatic analysis of orange protein (OR) gene from Nicotiana tabacum

    LEI Bo(1); LU Kun(2); DING Fu-zhang(1); LIU Xiao-rong(2); HU Zhong-yi(1); PAN Wen-jie(1)
    (1) Guizhou Tobacco Science Research Institute, Guiyang, Guizhou, P.R. China; (2) College of Agronomy and Biothehnology of Southwest University, Chongqing, P.R. China
    Previous microarray results indicated that the orange protein (OR) gene in the carotenoid biosynthesis pathway showed significant differential expression in leaves between moderate and light aroma tobacco areas. In order to obtain the full-length sequence ...
  6. Tobacco Science & Technology, 2012, 1, p. 74-77., ISSN.1002-0861

    Effects of girdling on physical characters and smoking quality of flue-cured tobacco leaves

    YANG Chunyuan
    Guizhou Tobacco Science Research Institute/China Tobacco Southwest Agricultural Experiment Station, Guiyang 550081, P.R. China
    In order to improve cultivation practices and promote leaf quality, the stalk of tobacco plant was girdled after topping. The results showed that the girdling affected physical characters and smoking quality of flue-cured tobacco leaves greatly. The ...
  7. Tobacco Science & Technology, 2012, 10, p. 31-35, ISSN.1002-0861

    Comparison of main secondary metabolite contents in flue-cured tobacco leaves from different areas in Guizhou

    GENG Zhaoliang(1); FENG Yonggang(1); XIANG Zhangmin(1); ZHANG Jie(1); CAI Kai(1); ZHU Xianling(*2)
    (1) Guizhou Tobacco Research Institute, Guiyang 550081, P.R. China; (2) Research Center of Tobacco and Health, University of Science and Technology of China, Hefei 230051, P.R. China
    In order to compare the secondary metabolite contents in flue-cured leaves from different areas in Guizhou Province, the leaf samples of C3F and B2F grades of K326, Yunyan 85, Yunyan 87, Guiyan 1 and Guiyan 201 varieties were collected from Daozhen, ...
  8. Tobacco Science & Technology, 2012, 11, p. 74-78, ISSN.1002-0861

    Influences of arbuscular mycorrhizal fungi on phosphorus content in tobacco planting soil and nutrition absorption of flue-cured tobacco plants

    YAO Juan(1); WANG Maosheng(2); WANG Feng(*1,3); XU Dongqing(2)
    (1) Agricultural College of Guizhou University, Guiyang 550025, P.R. China; (2) Guizhou Tobacco Science Institute, Guiyang 550081, P.R. China; (3) Qianxinan Prefecture Tobacco Company of Guizhou Province, Xingyi 562400, Guizhou, P.R. China
    In order to study the mycorrhizal effects of mycorrhizal seedling after transplanting, three nitrogen (N) levels, 45, 67.5 and 90 kg/ha, were applied in field experiments and the effects of mycorrhizal seedlings on phosphorus (P) content in soil and N and ...
  9. Tobacco Science & Technology, 2012, 3, p. 34-37., ISSN.1002-0861

    Cloning and sequence analysis of T-zds1 gene from flue-cured tobacco

    HU Zhongyi(1) ; LEI Bo(1) ; LIU Dong(2) ; CAI Liuti(1)
    (1) Guizhou Tobacco Research Institute, Guiyang 550081, P.R. China; (2) College of Agriculture, Yangtze University, Jingzhou 434023, Hubei, P.R. China
    Homology cloning and in silico cloning method were used to clone the full length cDNA sequence of tobacco zeta carotene dehydrogenase gene (named T-zds 1) from flue-cured tobacco cultivar, Jiucaiping2, and the primary and secondary structures of its ...