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14 results

  1. Tobacco Science & Technology, 2012, 1, p. 74-77., ISSN.1002-0861

    Effects of girdling on physical characters and smoking quality of flue-cured tobacco leaves

    YANG Chunyuan
    Guizhou Tobacco Science Research Institute/China Tobacco Southwest Agricultural Experiment Station, Guiyang 550081, P.R. China
    In order to improve cultivation practices and promote leaf quality, the stalk of tobacco plant was girdled after topping. The results showed that the girdling affected physical characters and smoking quality of flue-cured tobacco leaves greatly. The ...
  2. Tobacco Science & Technology, 2012, 10, p. 31-35, ISSN.1002-0861

    Comparison of main secondary metabolite contents in flue-cured tobacco leaves from different areas in Guizhou

    GENG Zhaoliang(1); FENG Yonggang(1); XIANG Zhangmin(1); ZHANG Jie(1); CAI Kai(1); ZHU Xianling(*2)
    (1) Guizhou Tobacco Research Institute, Guiyang 550081, P.R. China; (2) Research Center of Tobacco and Health, University of Science and Technology of China, Hefei 230051, P.R. China
    In order to compare the secondary metabolite contents in flue-cured leaves from different areas in Guizhou Province, the leaf samples of C3F and B2F grades of K326, Yunyan 85, Yunyan 87, Guiyan 1 and Guiyan 201 varieties were collected from Daozhen, ...
  3. Tobacco Science & Technology, 2012, 11, p. 74-78, ISSN.1002-0861

    Influences of arbuscular mycorrhizal fungi on phosphorus content in tobacco planting soil and nutrition absorption of flue-cured tobacco plants

    YAO Juan(1); WANG Maosheng(2); WANG Feng(*1,3); XU Dongqing(2)
    (1) Agricultural College of Guizhou University, Guiyang 550025, P.R. China; (2) Guizhou Tobacco Science Institute, Guiyang 550081, P.R. China; (3) Qianxinan Prefecture Tobacco Company of Guizhou Province, Xingyi 562400, Guizhou, P.R. China
    In order to study the mycorrhizal effects of mycorrhizal seedling after transplanting, three nitrogen (N) levels, 45, 67.5 and 90 kg/ha, were applied in field experiments and the effects of mycorrhizal seedlings on phosphorus (P) content in soil and N and ...
  4. Tobacco Science & Technology, 2012, 3, p. 34-37., ISSN.1002-0861

    Cloning and sequence analysis of T-zds1 gene from flue-cured tobacco

    HU Zhongyi(1) ; LEI Bo(1) ; LIU Dong(2) ; CAI Liuti(1)
    (1) Guizhou Tobacco Research Institute, Guiyang 550081, P.R. China; (2) College of Agriculture, Yangtze University, Jingzhou 434023, Hubei, P.R. China
    Homology cloning and in silico cloning method were used to clone the full length cDNA sequence of tobacco zeta carotene dehydrogenase gene (named T-zds 1) from flue-cured tobacco cultivar, Jiucaiping2, and the primary and secondary structures of its ...
  5. Tobacco Science & Technology, 2012, 4, p. 60-66, ISSN.1002-0861

    Cloning and bioinformatics analysis of orange protein (OR) gene from Nicotiana tabacum

    LEI Bo(1); LU Kun(2); DING Fuzhang(1); LIU Xiaorong(2); HU Zhongyi(1); PAN Wenjie(1)
    (1) Guizhou Tobacco Research Institute, Guiyang 550081, P.R. China; (2) College of Agronomy and Biotechnology of Southwest University, Chongqing 400715, P.R. China
    In order to obtain the full-length sequence of NtOR and reveal its biological function, the full-length cDNA and genomic DNA sequences of NtOR were cloned from cv. K326 using homologous cloning method, and logged in GenBank (accession numbers JN379458 and ...
  6. Tobacco Science & Technology, 2012, 8, p. 54-59, 65, ISSN.1002-0861

    Correlation analysis between nicotine content and medium and trace element contents in flue-cured tobacco leaves from different stalk positions

    CHEN Wei(1); TANG Yuanju(1); PAN Wenjie(1); LI Zhiyong(*2)
    (1) Guizhou Tobacco Research Institute, Guiyang 550081, P.R. China; (2) Guizhou Provincial Tobacco Company, Guiyang 550003, P.R. China
    The relationships between nicotine content and the contents of medium and trace elements in flue-cured tobacco leaves from different stalk positions were analyzed with descriptive statistics analysis, stepwise regression analysis and path analysis. The ...
  7. Tobacco Science & Technology, 2012, 8, p. 70-74, ISSN.1002-0861

    Cloning and sequence analysis of β-carotene hydroxylase gene in Nicotiana tabacum

    LIN Shifeng; WANG Rengang; Wang Yi; REN Xueliang*
    Guizhou Tobacco Research Institute, Guiyang 550081, P.R. China
    In order to effectively modulate the anabolism of carotenoid in tobacco, a β-carotene hydroxylase (BCH) gene related to plant carotenoid anabolism was cloned from Nicotiana tabacum. A cDNA sequence of encoded BCH gene was isolated from tobacco cv. K326 by ...
  8. Tobacco Science & Technology, 2012, 8, p. 79-83, ISSN.1002-0861

    Preliminary analysis of soil microbial diversity in tobacco bacterial wilt disease nursery

    CAI Liuti(1,2); SHANG Shenghua(1); HU Zhongyi(1); WANG Lili(2); LIU Yanxia(2); WANG Hancheng(1); YANG Xingming(2); SHI Junxiong(*1)
    (1) Guizhou Tobacco Research Institute, Guiyang 550081, P.R. China; (2) College of Resource and Environmental Science, Nanjing Agricultural University, Nanjing 210095, P.R. China
    To prevent and control tobacco bacterial wilt disease with antagonistic bacterium against Ralstonia solanacearum, the microbial diversity in soil of tobacco bacterial wilt disease nursery was preliminarily analyzed by means of conservative fragment ...
  9. Tobacco Science & Technology, 2011, 1, p. 60-65., ISSN.1002-0861

    Comparison of major components in leaf trichome exudate of flue-cured tobacco grown under different climatic conditions

    ZHU XIAN-LING(1); PAN WEN-JIE(2); CHEN YI(2); CHEN WEI(2); LI ZHANG-HAI(1); ZHANG JI-LI(1); XU ZENG-HAN(1); LI HONG-XUN(2)
    1. Research Center of Tobacco and Health, University of Science and Technology of China, Hefei 230051, P.R. China; 2. Guizhou Tobacco Science Research Institute, Guiyang 550023, P.R. China
    In order to find out the effects of climatic factors on leaf trichome exudates, a field experiment on flue-cured tobacco cv. Yun 85 was conducted at Longgang of Kaiyang County, Weining County and Tianzhu County in Guizhou Province. Ripe leaves harvested ...
  10. Tobacco Science & Technology, 2011, 2, p. 58-63., ISSN.1002-0861

    Cloning and sequence analysis of T-psy1 gene from flue-cured tobacco

    CAI LIU-TI(1); JIANG GUANG-HUA(2); LEI BO(1); HU ZHONG-YI(1)
    1 .Guizhou Tobacco Research Institute, Guiyang 550018, P.R. China; 2. Guizhou Provincial Tobacco Company, Guiyang 550003, P.R. China
    Adopting a homology cloning method, the full length cDNA sequence of tobacco phytoene synthase gene (named T-psy1) was cloned from flue-cured tobacco cv. K326, the T-psy1 gene GenBank accession number HM345582, included 1521bp, with coding protein ...