1. Tobacco Science & Technology, 2012, 4, p. 72-75, ISSN.1002-0861

    Structural characteristic analysis and mRNA expression of Cathepsin B gene in Nicotiana tabacum

    ZHAO Min(1); WANG Changguo(1); LIU Jin(2); KOU Mingyu(1); LI Ning(1); WU Yan(1); DAI Ya(*1); XIA Qingyou(*2,3)
    (1) Technology Center of China Tobacco Chuanyu Industrial Co., Ltd., Chengdu 610066, P.R. China; (2) College of Biotechnology, Southwest University, Chongqing 400715, P.R. China; (3) Institute of Agronomy and Life Science, Chongqing University, Chongqing 400030, P.R. China
    In order to clarify the function of Cathepsin B gene (NbCathB) in Nicotiana tabacum, the sequence of NbCathB was analyzed. The NbCathB ORF (Open Read Frame) was 951 bp in length and encoded 316 amino acids with the predicted molecular weight of 35.2 kD ...
  2. Tobacco Science & Technology, 2012, 7, p. 72-75, ISSN.1002-0861

    Analysis of differential protein in tobacco leaves sprayed with microbial agent MP

    ZHAO Min(1); WANG Changguo(1); LIU Jin(2); KOU Mingyu(1); LI Ning(1); WU Yan(1); DAI Ya(*1); XIA Qingyou(*2)
    (1) Technology Center, China Tobacco Chuanyu Industrial Co., Ltd., Chengdu 610066, P.R. China; (2) College of Biotechnology, Southwest University, Chongqing 400715, P.R. China
    In order to find out the effects of Microbial agent MP (Bacillus magaterium) spraying on the quality of tobacco leaves, two-dimensional gel electrophoresis (2-DE) was used to analyze the differences of protein expression between tobacco leaves sprayed ...
  3. Tobacco Science & Technology, 2012, 9, p. 75-81, ISSN.1002-0861

    Cloning and sequence analysis of carotenoid cleavage dioxygenase gene from Nicotiana tabacum

    GAO Junping(1); WANG Genhong(1,2); CHENG Tingcai(1,2); DUAN Jun(1,2); XIA Qingyou(*2)
    (1) Institute of Agriculture and Life Science, Chongqing University, Chongqing 400044, P.R. China; (2) State Key Laboratory of Silkworm Genome Biology, Southwest University, Chongqing 400715, P.R. China
    In order to reveal the function of carotenoid cleavage dioxygenases (CCDs) in the growth and development of tobacco plants, a CCD4 (NtCCD4) gene was cloned from Samsun oriental tobacco by RT-PCR. According to sequence analysis, with a coding sequence of ...