1. Tobacco Science & Technology, 2012, 3, p. 34-37., ISSN.1002-0861

    Cloning and sequence analysis of T-zds1 gene from flue-cured tobacco

    HU Zhongyi(1) ; LEI Bo(1) ; LIU Dong(2) ; CAI Liuti(1)
    (1) Guizhou Tobacco Research Institute, Guiyang 550081, P.R. China; (2) College of Agriculture, Yangtze University, Jingzhou 434023, Hubei, P.R. China
    Homology cloning and in silico cloning method were used to clone the full length cDNA sequence of tobacco zeta carotene dehydrogenase gene (named T-zds 1) from flue-cured tobacco cultivar, Jiucaiping2, and the primary and secondary structures of its ...
  2. Tobacco Science & Technology, 2012, 4, p. 60-66, ISSN.1002-0861

    Cloning and bioinformatics analysis of orange protein (OR) gene from Nicotiana tabacum

    LEI Bo(1); LU Kun(2); DING Fuzhang(1); LIU Xiaorong(2); HU Zhongyi(1); PAN Wenjie(1)
    (1) Guizhou Tobacco Research Institute, Guiyang 550081, P.R. China; (2) College of Agronomy and Biotechnology of Southwest University, Chongqing 400715, P.R. China
    In order to obtain the full-length sequence of NtOR and reveal its biological function, the full-length cDNA and genomic DNA sequences of NtOR were cloned from cv. K326 using homologous cloning method, and logged in GenBank (accession numbers JN379458 and ...
  3. CORESTA Meeting, Agronomy/Phytopathology, 2011, Santiago, APOST 05

    Cloning and gene structure analysis of T-psy1 from flue-cured tobacco

    CAI Liuti(1); HU Zhongyi(1); LEI Lu(1); LI Jixin(2)
    (1) Guizhou Tobacco Research Institute, Guizhou Provincial Tobacco Company, Guiyang, P.R China; (2) Guizhou Provincial Tobacco Company, Guiyang, P.R China
    Carotenoid is an important precursor of tobacco aroma, and phytoene synthase is one of the key enzymes in the pathway of aroma precursors biosynthesis and accumulation in tobacco. Based on the homology cloning method, the cDNA and DNA sequence of the ...