1. CORESTA Meeting, Smoke Science/Product Technology, 2017, Kitzbühel, STPOST 12

    Study on vaporisation temperature of electronic cigarette

    CUI Huapeng; ZHAO Le; FAN Meijuan; LIU Shaofeng; CHEN Li; CAI Junlan; QIN Yaqiong; DING Xue; JIANG Zhicai
    (1) Zhengzhou Tobacco Research Institute of CNTC, Zhengzhou, Henan, P.R. China; (2) China Tobacco Zhejiang Industrial Co., Ltd, Hangzhou, Zhejiang, P.R. China
    The vaporisation temperature of electronic cigarettes (e-cigarettes) affects the release and migration of harmful components in e-cigarettes greatly. Therefore a method for measuring the vaporisation temperature was established based on a thermocouple to ...
  2. TSRC, Tob. Sci. Res. Conf., 2016, 70, abstr. 80

    Volatile components release characteristics of tobacco smoke under heating conditions

    ZENG Shitong(1); CUI Kai(1); LIU Shan(1); TANG Peipei(1); SUN Shihao(1); ZONG Yongli(1); ZHANG Jianxun(1); HU Anfu(2); YANG Jun(2)
    (1) Zhengzhou Tobacco Research Institute of CNTC, Zhengzhou, China; (2) China Tobacco Zhejiang Industrial Co., Ltd, Zhejiang, China
    In order to investigate volatile components release characteristics of tobacco smoke under heating conditions, seven different tobacco samples including flue-cured tobacco (B2F, C3F, X2F), oriental tobacco (B1, B2) and burley tobacco (B2F, C3F) were ...
  3. CORESTA Meeting, Agronomy/Phytopathology, 2015, Izmir, Turkey, AP 41

    Effects of different growth temperatures on alkaloid content and nicotine metabolism in roots, stems and leaves of tobacco (Nicotiana tabacum L.) plants

    JIN Y.F.(1); WANG S.S.(1); LI J.Y.(2); PANG T.(2); ZHANG J.B.(1); GONG M.(1)
    (1) School of Life Sciences, Yunnan Normal University, Kunming, P.R. China; (2) Yunnan Academy of Tobacco Agricultural Sciences, Kunming, P.R. China
    Alkaloids, especially nicotine content, significantly influences quality and flavour types of tobacco leaves, which is differentially regulated by various environmental conditions. To investigate the effects of different growth temperature on alkaloids ...
  4. CORESTA Congress, Sapporo, 2012, Agronomy/Phytopathology Groups, APPOST 09

    Cloning and bioinformatic analysis of orange protein (OR) gene from Nicotiana tabacum

    LEI Bo(1); LU Kun(2); DING Fu-zhang(1); LIU Xiao-rong(2); HU Zhong-yi(1); PAN Wen-jie(1)
    (1) Guizhou Tobacco Science Research Institute, Guiyang, Guizhou, P.R. China; (2) College of Agronomy and Biothehnology of Southwest University, Chongqing, P.R. China
    Previous microarray results indicated that the orange protein (OR) gene in the carotenoid biosynthesis pathway showed significant differential expression in leaves between moderate and light aroma tobacco areas. In order to obtain the full-length sequence ...
  5. Tobacco Science & Technology, 2012, 2, p. 43-46., ISSN.1002-0861

    Determination of NNN and its metabolites in rabbit blood with HPLC-MS/MS

    ZHAO Beibei(1); WANG Sheng(1); WANG Juan(2); WANG Zhaoyu(3); ZHANG Jianxun(1)
    (1) Zhengzhou Tobacco Research Institute of CNTC, Zhengzhou 450001, P.R. China; (2) Quality Supervision & Test Station of Hainan Tobacco Corporation, Haikou 571100, P.R. China; (3) Quality Supervision & Test Station of Zhejiang Tobacco Corporation, Hangzhou 310001, P.R. China
    In order to accurately determine N-nitroso nornicotine (NNN) and its metabolites in rabbit blood, a high-performance liquid chromatography/tandem mass spectrometry method was established by optimizing chromatographic conditions and mass spectrometric ...
  6. Tobacco Science & Technology, 2012, 3, p. 38-47., ISSN.1002-0861

    Effects of ambient temperature and relative humidity on migration behavior of tobacco flavors in cigarettes

    BAI Xinliang(1) ; HU Jun(1), WANG Jun(2); ZHOU Jianhua(2); CHU Guohai(2); HU Youchi(1); ZENG Shitong(1); ZHAO Mingyue(1); YANG Jun(2)
    (1) Zhengzhou Tobacco Research Institute of CNTC, Zhengzhou 450001, P.R. China; (2) China Tobacco Zhejiang Industrial Co., Ltd., Hangzhou 310009, P.R. China
    In order to investigate the effects of ambient temperature and relative humidity (RH) on the migration behavior of tobacco flavors in cigarette, the migration ratios of flavors to mainstream smoke (MS) and their retention ratios by filter tips were ...
  7. Tobacco Science & Technology, 2012, 3, p. 79-82, ISSN.1002-0861

    Identification of Ralstonia solanacearum isolated from Burley planted soil and its biovars

    WU Weiling(1); ZHANG Dinggui(2); YANG Shuiying(3); WANG Han(1); LI Hong(1); LI Zhenlun(1)
    (1) College of Resource and Environment of Southwest University, Chongqing 400716, P.R. China; (2) Dazhou Branch of Sichuan Province Tobacco Company, Dazhou 635006, Sichuan, P.R. China; (3) College of Plant Protection of Southwest University, Chongqing 400716, P.R. China
    In order to establish a method which is rapid and low in cost, for isolating and identifying the viable cells of Ralstonia solanacearum E.F. Smith (R. solanacearum) in burley planted soil, a semiselective medium (PCCG) combined with polymerase chain ...
  8. Tobacco Science & Technology, 2012, 4, p. 60-66, ISSN.1002-0861

    Cloning and bioinformatics analysis of orange protein (OR) gene from Nicotiana tabacum

    LEI Bo(1); LU Kun(2); DING Fuzhang(1); LIU Xiaorong(2); HU Zhongyi(1); PAN Wenjie(1)
    (1) Guizhou Tobacco Research Institute, Guiyang 550081, P.R. China; (2) College of Agronomy and Biotechnology of Southwest University, Chongqing 400715, P.R. China
    In order to obtain the full-length sequence of NtOR and reveal its biological function, the full-length cDNA and genomic DNA sequences of NtOR were cloned from cv. K326 using homologous cloning method, and logged in GenBank (accession numbers JN379458 and ...
  9. Tobacco Science & Technology, 2012, 4, p. 72-75, ISSN.1002-0861

    Structural characteristic analysis and mRNA expression of Cathepsin B gene in Nicotiana tabacum

    ZHAO Min(1); WANG Changguo(1); LIU Jin(2); KOU Mingyu(1); LI Ning(1); WU Yan(1); DAI Ya(*1); XIA Qingyou(*2,3)
    (1) Technology Center of China Tobacco Chuanyu Industrial Co., Ltd., Chengdu 610066, P.R. China; (2) College of Biotechnology, Southwest University, Chongqing 400715, P.R. China; (3) Institute of Agronomy and Life Science, Chongqing University, Chongqing 400030, P.R. China
    In order to clarify the function of Cathepsin B gene (NbCathB) in Nicotiana tabacum, the sequence of NbCathB was analyzed. The NbCathB ORF (Open Read Frame) was 951 bp in length and encoded 316 amino acids with the predicted molecular weight of 35.2 kD ...