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16 results

  1. Tobacco Science & Technology, 2012, 2, p. 43-46., ISSN.1002-0861

    Determination of NNN and its metabolites in rabbit blood with HPLC-MS/MS

    ZHAO Beibei(1); WANG Sheng(1); WANG Juan(2); WANG Zhaoyu(3); ZHANG Jianxun(1)
    (1) Zhengzhou Tobacco Research Institute of CNTC, Zhengzhou 450001, P.R. China; (2) Quality Supervision & Test Station of Hainan Tobacco Corporation, Haikou 571100, P.R. China; (3) Quality Supervision & Test Station of Zhejiang Tobacco Corporation, Hangzhou 310001, P.R. China
    In order to accurately determine N-nitroso nornicotine (NNN) and its metabolites in rabbit blood, a high-performance liquid chromatography/tandem mass spectrometry method was established by optimizing chromatographic conditions and mass spectrometric ...
  2. Tobacco Science & Technology, 2012, 3, p. 38-47., ISSN.1002-0861

    Effects of ambient temperature and relative humidity on migration behavior of tobacco flavors in cigarettes

    BAI Xinliang(1) ; HU Jun(1), WANG Jun(2); ZHOU Jianhua(2); CHU Guohai(2); HU Youchi(1); ZENG Shitong(1); ZHAO Mingyue(1); YANG Jun(2)
    (1) Zhengzhou Tobacco Research Institute of CNTC, Zhengzhou 450001, P.R. China; (2) China Tobacco Zhejiang Industrial Co., Ltd., Hangzhou 310009, P.R. China
    In order to investigate the effects of ambient temperature and relative humidity (RH) on the migration behavior of tobacco flavors in cigarette, the migration ratios of flavors to mainstream smoke (MS) and their retention ratios by filter tips were ...
  3. Tobacco Science & Technology, 2012, 3, p. 79-82, ISSN.1002-0861

    Identification of Ralstonia solanacearum isolated from Burley planted soil and its biovars

    WU Weiling(1); ZHANG Dinggui(2); YANG Shuiying(3); WANG Han(1); LI Hong(1); LI Zhenlun(1)
    (1) College of Resource and Environment of Southwest University, Chongqing 400716, P.R. China; (2) Dazhou Branch of Sichuan Province Tobacco Company, Dazhou 635006, Sichuan, P.R. China; (3) College of Plant Protection of Southwest University, Chongqing 400716, P.R. China
    In order to establish a method which is rapid and low in cost, for isolating and identifying the viable cells of Ralstonia solanacearum E.F. Smith (R. solanacearum) in burley planted soil, a semiselective medium (PCCG) combined with polymerase chain ...
  4. Tobacco Science & Technology, 2012, 4, p. 60-66, ISSN.1002-0861

    Cloning and bioinformatics analysis of orange protein (OR) gene from Nicotiana tabacum

    LEI Bo(1); LU Kun(2); DING Fuzhang(1); LIU Xiaorong(2); HU Zhongyi(1); PAN Wenjie(1)
    (1) Guizhou Tobacco Research Institute, Guiyang 550081, P.R. China; (2) College of Agronomy and Biotechnology of Southwest University, Chongqing 400715, P.R. China
    In order to obtain the full-length sequence of NtOR and reveal its biological function, the full-length cDNA and genomic DNA sequences of NtOR were cloned from cv. K326 using homologous cloning method, and logged in GenBank (accession numbers JN379458 and ...
  5. Tobacco Science & Technology, 2012, 4, p. 72-75, ISSN.1002-0861

    Structural characteristic analysis and mRNA expression of Cathepsin B gene in Nicotiana tabacum

    ZHAO Min(1); WANG Changguo(1); LIU Jin(2); KOU Mingyu(1); LI Ning(1); WU Yan(1); DAI Ya(*1); XIA Qingyou(*2,3)
    (1) Technology Center of China Tobacco Chuanyu Industrial Co., Ltd., Chengdu 610066, P.R. China; (2) College of Biotechnology, Southwest University, Chongqing 400715, P.R. China; (3) Institute of Agronomy and Life Science, Chongqing University, Chongqing 400030, P.R. China
    In order to clarify the function of Cathepsin B gene (NbCathB) in Nicotiana tabacum, the sequence of NbCathB was analyzed. The NbCathB ORF (Open Read Frame) was 951 bp in length and encoded 316 amino acids with the predicted molecular weight of 35.2 kD ...
  6. Tobacco Science & Technology, 2012, 7, p. 17-23, ISSN.1002-0861

    Correlation analysis between appearance quality and sensory quality of Henan flue-cured tobacco

    YAN Hongyang(1); YAN Hongxi(2); JI Songyi(*3,4); HAN Ming(5); LEI Jinshan(6); YAN Keyu(7)
    (1) Staff Dev. Inst. of CNTC, Zhengzhou 450008, PRC; (2) Hangzhou Cig. Factory, CNTC Zhejiang Ind. Co., Ltd., Hangzhou 310008, PRC; (3) CNTC Yunnan Kunchuan Reascend Science & Technology Co., Ltd., Kunming 650106, PRC; (4) Yunnan Reascend Tobacco Technology (Group) Co., Ltd., Kunming 650106, PRC; (5) Anyang Cig. Factory, CNTC Henan Ind. Co., Ltd., Anyang 455004, PRC; (6) Changcheng Cigar Factory, CNTC Chuanyu Ind. Co., Ltd., Shifang 618400, PRC; (7) Zhengzhou Uni. of Light Industry, Zhengzhou 450002, PRC
    Taking a complete set of grading national standard samples of Henan flue-cured tobacco duplicated from the national standards as experimental material, the appearance and sensory qualities of each grade sample were quantitatively evaluated according to ...
  7. Tobacco Science & Technology, 2012, 7, p. 72-75, ISSN.1002-0861

    Analysis of differential protein in tobacco leaves sprayed with microbial agent MP

    ZHAO Min(1); WANG Changguo(1); LIU Jin(2); KOU Mingyu(1); LI Ning(1); WU Yan(1); DAI Ya(*1); XIA Qingyou(*2)
    (1) Technology Center, China Tobacco Chuanyu Industrial Co., Ltd., Chengdu 610066, P.R. China; (2) College of Biotechnology, Southwest University, Chongqing 400715, P.R. China
    In order to find out the effects of Microbial agent MP (Bacillus magaterium) spraying on the quality of tobacco leaves, two-dimensional gel electrophoresis (2-DE) was used to analyze the differences of protein expression between tobacco leaves sprayed ...
  8. Tobacco Science & Technology, 2012, 9, p. 47-50, ISSN.1002-0861

    Application of electronic nose in quality control of tobacco flavor

    JIANG Jian(1); YANG Jun(1); HUANG Fangfang(1); TANG Cunxiao(2); HE Jinzhe(2); SUN Peilong(2)
    (1) China Tobacco Zhejiang Industrial Co., Ltd., Hangzhou 310009, P.R. China; (2) Food Department, College of Biology and Environmental Engineering, Zhejiang University of Technology, Hangzhou 310014, P.R. China
    Nine tobacco flavor samples were detected by an electronic nose, the obtained data were analyzed with principal component analysis (PCA) and discriminant factor analysis (DFA). The models of quality control were established, and the quality consistency of ...
  9. Tobacco Science & Technology, 2012, 9, p. 58-62, ISSN.1002-0861

    Selective removal of hydrogen cyanide from cigarette smoke by combined filter of coptis alkaloid

    WU Jinfeng(1,2); LI Xuegang(1); WANG Peng(3); PENG Zhong(3); LAN Zhongyu(3); ZHENG Lifeng(2)
    (1) College of Pharmaceutical Sciences, Southwest University, Chongqing 400715, P.R. China; (2) Chongqing Cigarette Factory, Chongqing Tobacco Industrial Co., Ltd., China Tobacco Chuanyu Industrial Co., Ltd., Chongqing 400061, P.R. China; (3) Department of Technology, Chongqing Tobacco Industrial Co., Ltd., China Tobacco Chuanyu Industrial Co., Ltd., Chongqing 400060, P.R. China
    Coptis alkaloids were extracted from rhizome of Coptis chinensis rich in quaternary ammonium hydroxide and added into cigarette filter. On the basis of the reduction of hydrogen cyanide delivery in cigarette smoke, the way of coptis alkaloid adding into ...