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2 results

  1. CORESTA Congress, Quebec, 2014, Smoke Science/Product Technology Groups, STPOST 21

    Evaluating the genotoxicity of tobacco smoke-derived aerosols using the flow cytometry-based in vitro micronucleus assay

    POULEAU B.; SMART D.J.; McHUGH D.
    Philip Morris International R&D, Philip Morris Products S.A., Neuchâtel, Switzerland
    The in vitro micronucleus (MN) assay is used to assess the genotoxic potential of a test substance by measuring the induction of micronuclei in cultured mammalian cells. The flow cytometry-based assay (in vitro MicroFlow ® kit, Litron Laboratories, USA) ...
  2. CORESTA Congress, Quebec, 2014, Smoke Science/Product Technology Groups, STPOST 23

    Quantitative evaluation of the mutagenicity induced by tobacco smoke-derived total particulate matter using the mouse lymphoma assay

    DEMOUTE A-L.; AL-JOBORI O.; POULEAU B.; SMART D.J.; McHUGH D.
    Philip Morris International R&D, Philip Morris Products S.A., Neuchâtel, Switzerland
    The mouse lymphoma assay (MLA) is a regulatory in vitro test deployed to evaluate the mutagenicity of a test substance via exposure of the thymidine kinase locus in L5178Y mouse lymphoma cells. As part of a proficiency study, we evaluated the mutagenicity ...