Search Results

9 results

  1. Tobacco Science & Technology, 2012, 10, p. 77-80, ISSN.1002-0861

    Adsorption purification of TMV antiserum and detection application

    LUO Zhaopeng(1); WEI Chunyang(1); LI Naihui(2); YANG Jun(1); JIN Dawei(1); JIN Lifeng(1); LI Feng(1); LIN Fucheng(1)
    (1) Zhengzhou Tobacco Research Institute of CNTC, Zhengzhou 450001, P.R. China; (2) Linyi Tobacco Limited Company of Shandong Province, Linyi 276003, Shandong, P.R. China
    For the detection of TMV in tobacco, antiserum was prepared by injecting purified TMV virion into mouse and the titer of antiserum was determined after adsorption purification, an ELISA analysis system was established basing on the in-house TMV antiserum. ...
  2. Tobacco Science & Technology, 2012, 12, p. 72-77, ISSN.1002-0861

    Serological identification of viruses infecting tobacco in major tobacco growing areas of China

    LUO Zhaopeng; YANG Jun; WANG Ran; LI Feng; JIN Lifeng; WEI Chunyang; LIN Fucheng
    Zhengzhou Tobacco Research Institute of CNTC, Zhengzhou 450001, P.R. China
    In order to better understand the occurrence of tobacco virus diseases in China status quo, 409 symptomatic tobacco samples were collected from 13 major tobacco growing areas of China in 2010 and 2011. Tobacco mosaic virus (TMV), cucumber mosaic virus ...
  3. Tobacco Science & Technology, 2012, 3, p. 67-74, ISSN.1002-0861

    Variance analysis of carotenoid and its degradation product contents in flue-cured tobacco of different appearance

    XI Yuanxiao(1,2); LIU Yang(1); LI Feng(1); YANG Jun(1); WANG Xinmin(1); WEI Chunyang(1); SONG Jizhen(1)
    (1) Zhengzhou Tobacco Research Institute of CNTC, Zhengzhou 450001, P.R. China; (2) Shandong Weifang Tobacco Company, Weifang 261061, Shandong, P.R. China
    The contents of carotenoid and its degradation products were determined by HPLC and GC/MS, respectively. The results showed that: 1) The contents of lutein, β-carotene and carotenoid increased with the darkening of leaf color, decreased with the increase ...
  4. Tobacco Science & Technology, 2012, 5, p. 60-65, 78, ISSN.1002-0861

    Cloning and characterization of a new gene encoding geranylgeranyl pyrophosphate synthase from Nicotiana tabacum

    LI Feng(1); LI Ming(2); JIN Lifeng(1); LUO Zhaopeng(1); YANG Jun(1); LIN Fucheng(*1)
    (1) Zhengzhou Tobacco Research Institute of CNTC, Zhengzhou 450001, P.R. China; (2) Institute of Botany, CAS, Beijing 100093, P.R. China
    A new cDNA sequence encoding GGPPS (designated as Ntggpps3) from tobacco (Nicotiana tabacum) was cloned by RACE techniques and assayed by bioinformatics. The cDNA sequence of Ntggpps3 was of 1251 bp with an open reading frame (ORF) of 1092 bp encoding 363 ...
  5. CORESTA Meeting, Agronomy/Phytopathology, 2011, Santiago, PPOST 04

    Molecular variation characterization of the genome 5' terminal region of PVY isolated from tobacco in China

    YANG Jun; LUO Zhao-peng; JIN Li-feng; ZHAI Niu; LI Feng; XU Ya-long; ZHOU Hui-na; LIN Fu-cheng
    Zhengzhou Tobacco Research Institute, Zhegzhou, Henan, P.R. China.
    Tobacco viral diseases, of which most are RNA viruses, are a category of infectious diseases that are distributed widely and occur severely. The natural variation (mutation) of tobacco viruses proceed quickly, and cause considerable difficulty for ...
  6. Tobacco Science & Technology, 2010, 10, p. 48-51., ISSN.1002-0861

    Grey incidence analysis on major appearance traits and smoking quality of flue-cured tobacco

    WEI CHUN-YANG(1); LUO ZHAO-PENG(1); LI FENG(1); LI WEI-GUAN(2); YANG MING-FENG(3); LIANG BAO-DE(4); WANG XIN-MIN(1); SONG JI-ZHEN(1)
    1. Zhengzhou Tobacco Research Institute of CNTC, Zhengzhou 450001, P.R. China; 2. Xiangcheng Branch of Xuchang Tobacco Company, Xiangcheng 461700, Henan, P.R. China; 3. Technology Center of China Tobacco Shandong Industrial Corporation, Qingdao 266101, Shandong, P.R. China
    Based on the evaluation data of appearance and smoking quality of flue-cured tobacco samples from main tobacco growing areas in China from 2004 to 2007, grey incidence analysis was performed. The results showed that for upper leaves, color intensity, leaf ...
  7. Tobacco Science & Technology, 2010, 12, p. 61-65., ISSN.1002-0861

    Classification and evaluation of appearance quality of flue-cured tobacco based on Eigenvector

    WEI CHUN-YANG; LI FENG; ZHANG SHI-XIANG; CAI XIAN-JIE; WANG XIN-MIN; SONG JI-ZHEN
    Zhengzhou Tobacco Research Institute of CNTC, Zhengzhou 450001, P.R. China
    In order to better evaluate the appearance quality of flue-cured tobacco, the Eigenvectors of flue-cured tobacco appearance quality were introduced and the classification method of appearance quality on the basis of the quantified evaluation system of ...
  8. Tobacco Science & Technology, 2010, 6, p. 73-76., ISSN.1002-0861

    Preliminary screening of bioactive substances from plants against tobacco mosaic virus

    JIN GUANG-HUI; YIN QI-SHENG; YANG JUN; LI FENG; JIN LI-FENG; LUO ZHAO-PENG; WANG XIN-MIN
    Zhengzhou Tobacco Research Institute of CNTC, Zhengzhou 450001, P.R. China
    The antiviral activities against TMV of crude protein extracts from 18 plants of 13 families were determined by local lesion assay. The results showed that the inhibition effects of different protein extracts on TMV infected Nicotiana glutinosa were ...
  9. Tobacco Science & Technology, 2010, 7, p. 58-61., ISSN.1002-0861

    Molecular identification of tobacco mosaic disease

    LUO CHAO-PENG; YANG JUN; JIN LI-FENG; LI FENG; WANG XIN-MIN; JIN GUANG-HUI; SONG JI-ZHEN
    Zhengzhou Tobacco Research Institute of CNTC, Zhengzhou 450001, P.R. China
    In order to establish a system for molecular identification of tobacco virus diseases, primers for TMV, CMV and PVY detection were used in RT-PCR for TMV samples, and the genetic sequence of the amplified product was analyzed at nucleic acid molecular ...