Search Results

18 results

  1. CORESTA Meeting, Agronomy/Phytopathology, 2017, Santa Cruz do Sul, AP 39

    Identification of genetic determinants controlling cadmium accumulation in tobacco

    BERTRAND J.(1); JULIO E.(2); COTUCHEAU J.(2); DORLHAC DE BORNE F.(2); BERTHOMIEU P.(1)
    (1) UMR de Biochimie et Physiologie Moléculaire des Plantes, INRA/CNRS/Montpellier SupAgro/Université Montpellier, Montpellier, France; (2) Imperial Tobacco Limited, Leaf Research, La Tour, Bergerac, France
    Cadmium is a toxic heavy metal naturally absorbed and accumulated by plants. It is present in tobacco (Nicotiana tabacum) leaves and potentially in the smoke of cigarettes. Reducing the cadmium content in leaves of tobacco plants requires a better ...
  2. CORESTA Congress, Quebec, 2014, Agronomy/Phytopathology Groups, APPOST 03

    Agrobacterium T-DNA in Nicotiana tomentosiformis and other Nicotiana species

    CHEN K.(1); DORLHAC DE BORNE F.(2); SZEGEDI E.(3); OTTEN L.(1)
    (1) Department of Molecular Mechanisms of Phenotypic Plasticity, Institut de Biologie Moléculaire des Plantes du C.N.R.S., Strasbourg, France; (2) National Agricultural Research and Innovation Centre (NARIC), Research Institute for Viticulture and Enology, Experimental Station of Kecskemét, Hungary; (3) Imperial Tobacco Group, Institut du Tabac, Bergerac, France
    Agrobacterium rhizogenes induces growth of aberrant roots with bacterial T-DNA; some roots can spontaneously regenerate into plants carrying T-DNA fragments. T-DNAs contain genes that are expressed in plants which normally lead to root growth and to the ...
  3. CORESTA Congress, Edinburgh, 2010, SSPT 17

    GCxGC-MS characterisation of tobacco extracts: Classification and factors affecting the class maps

    VIAL J.; JOURNOUD P.; THIEBAUD A.; SASSIAT P.; TEILLET B.; CAHOURS X.; RIVALS I.
    ESPCI ParisTech, UMR CNRS UPMC PECSA, Laboratoire Sciences Analytiques, Bioanalytiques et Miniaturisation, Paris, France
    Comprehensive gas chromatography (GCxGC) appears as the preferred technique for the characterization of volatile compounds in tobacco samples. Indeed, GCxGC offers an enhanced separation power which results from the combination of two columns of different ...
  4. CORESTA Meeting, Smoke Science/Product Technology, Aix-en-Provence, 2009, SSPT 15

    Comparison of tobacco extracts: a strategy based on GCxGC-MS analysis and chemometric data processing

    VIAL J.; SASSIAT P.; THIÉBAUT D.; TEILLET B.; RIVALS I.; RUTLEDGE D.N.
    ESPCI ParisTech, CNRS PECSA 7195, Laboratoire Environnement et Chimie Analytique, Paris, France
    The extraction of tobacco, obtained either by Likens Nickerson or supercritical fluid extraction, generates samples that contain a high number of different compounds. The characterization and the comparison of these tobacco extracts, requires the use of a ...
  5. CORESTA Congress, Shanghai, 2008, SSPT 03

    Analysis of protein modifications induced by cigarette smoke

    LE FAOUDER P.; EMAMI I.; ROLANDO C.; TOKARSKI C.
    University of Sciences and Technologies of Lille, Laboratory of Organic and Macromolecular Chemistry, CNRS IMR 8009, Villeneuve d'Ascq, France
    We will present here work done on identification of protein modifications induced by cigarette smoke, particularly protein oxidation and nitration, using on-line nanoLC, ECD hyphenation on a FT-ICR instrument (Fourier Transform- Ion Cyclotron Resonance). ...
  6. CORESTA Congress, Shanghai, 2008, SSPT 07

    Identification and quantification of free radical content in cigarette smoke using nanoLC nano ESI FT-ICR MS/MS

    LE FAOUDER P.; TOKARSKI C.; EMAMI I.; ROLANDO C.
    University of Sciences and Technologies of Lille, Laboratory of Organic and Macromolecular Chemistry, CNRS IMR 8009, Villeneuve d'Ascq, France
    Cigarette smoke is a highly complex aerosol of several thousand chemical substances of which free radicals, short-life time molecules, are present in high concentration. In order to identify and quantify them, we have developed a new methodology based on ...
  7. CORESTA Congress, Shanghai, 2008, SSPTPOST 20

    Proteomics study of the influence of free radicals contents in cigarette smoke on culture cells

    LE FAOUDER P.; TOKARSKI C.; EMAMI I.; ROLANDO C.
    University of Sciences and Technologies of Lille, Laboratory of Organic and Macromolecular Chemistry, CNRS IMR 8009, Villeneuve d'Ascq, France
    The influence of free radicals from cigarette smoke on MCF-7 cell lines has been investigated in our laboratory using proteomics study. The work presented here will expose the comparison between untreated cells and cells incubated with conventional ...
  8. CORESTA Meeting, Agronomy/Phytopathology, Krakow, 2007, APPOST 02

    Rhizobacterial communities in soils suppressive or conducive to black root rot of tobacco analyzed by DGGE of biocontrol gene phlD and 16S rRNA microarray

    KYSELKOVÁ M.; FRAPOLLI M.; GRUNDMANN G.; DÉFAGO G.; MOËNNE-LOCCOZ Y.
    UMR CNRS 5557 Ecologie Microbienne, IFR 41 Bio-Environnement et Santé, Université Lyon 1, Villeurbanne, France
    In Morens (Switzerland), certain soils are suppressive to black root rot of tobacco, caused by the fungus Thielaviopsis basicola, whereas other soils are conducive (i.e. permit the spread of the disease). Suppressiveness to black root rot has been ...
  9. CORESTA Congress, Paris, 2006, PT 19

    Accelerated solvent extraction and supercritical fluid extraction coupled to comprehensive GCxGC for the analysis of volatile compounds in tobacco

    VIAL J.; THIEBAUT D.; SASSIAT P.; BELDEAN-GALEA M.S.; GOMEZ RAMOS M.J.; BOUZIGE M.
    ESPCI ParisTech, CNRS PECSA 7195, Laboratoire Environnement et Chimie Analytique, Paris, France
    Comprehensive GC×GC is a very powerful technique for complex sample analysis because a huge number of components can be separated according to carbon number and chemical classes. Two gas chromatography columns of different selectivities are connected via ...
  10. CORESTA Congress, Paris, 2006, SS 20

    Precise identification of free radicals in cigarette smoke by exact mass measurement using nano-LC, nanoESI FT-MS/MS

    ROLANDO C.; CANTAIS F.; EMAMI I.
    Université des Sciences et technologies de Lille, UMR CNRS 8009, Villeneuve d'Ascq cedex, France
    Free radicals are involved in a wide variety of ageing disease like cancer, cardiovascular disease and atherosclerosis. However, the molecular structure of free radicals that are contained in smoke is still the object of many controversies. This state ...