1. CORESTA Meeting, Agronomy/Phytopathology, 2021, Online, AP 54

    Effect of fermentation temperature on TSNA contents and aroma quality of cigar filler tobacco and microbial diversity analysis

    LI Jingjing(1); SHI Hongzhi(1); QIN Yanqing(2); ZHOU Jun(3); ZHAO Yuanyuan(1); ZHONG Qiu(4); LIU Deshui(3); WANG Jun(4); ZHANG Ruina(4)
    (1) College of Tobacco Science of Henan Agricultural University / Tobacco Harm Reduction Research Center of Hau, Zhengzhou, China; (2) Sichuan Provincial Tobacco Company, Chengdu, China; (3) Beijing Cigarette Factory of Shanghai Tobacco (Group) Co., Beijing, China; (4) Deyang Municipal Tobacco Company, Deyang, China
    Fermentation is indispensable for quality cigar tobacco production and fermentation temperature plays a pivotal role. Meanwhile, the fermentation process is also an important stage in the formation of tobacco specific nitrosamine (TSNA), and...
  2. CORESTA Congress, Online, 2020, Agronomy/Phytopathology Groups, AP 25

    Analysis of TSNAs and their relationship with alkaloids in cigar wrapper and filler tobaccos from different regions and varieties

    SHI Hongzhi(1); ZHOU Di(1); SUN Yuqi(1); WANG Jun(2); ZHOU Jun(3); ZHAO Yuanyuan(1); ZENG Dailong(4); QIN Yanqing(2); BAI Ruoshi(3); YANG Xingyou(2); LI Jingjing(1)
    (1) College of Tobacco Science of Henan Agricultural University / Tobacco Cultivation Key Laboratory of China Tobacco / Tobacco Harm Reduction Research Center of HAU, Zhengzhou, P.R. China; (2) Sichuan Tobacco Company, Chengdu, P.R. China; (3) Beijing Cigarette Factory of Shanghai Tobacco (Group) Co., Beijing, P.R. China; (4) China Tobacco Sichuan Industrial Co., Ltd, Great Wall Cigar Factory, Shifang, P.R. China
    Cigar tobacco production is growing rapidly as cigar consumption has been increasing recently in China. In order to be clear about the current status of tobacco-specific nitrosamine (TSNA) contents of cigar wrappers and fillers from different regions...
  3. CORESTA Congress, Online, 2020, Smoke Science/Product Technology Groups, ST 03

    Method development for the analysis of mono-carbonyl compounds in e-vapor products by LC-MS

    ZHU J.; HEREDIA A.; TWEEDY J.; TAYYARAH R.
    ITG Brands and Fontem USA, Greensboro, NC, U.S.A.
    At the 73rd Tobacco Science Research Conference (TSRC), we presented a novel method for quantitation by LC-MS of mono-carbonyl compounds in e-vapor product aerosol samples. The method is simple and robust with good recoveries. We have since extended...
  4. CORESTA Meeting, Agronomy/Phytopathology, 2019, Victoria Falls, APPOST 12

    Influences of plant growth promoting rhizobacteria (PGPR) inoculum combined with the reduction application of chemical fertilizer on the community structure of nosZ denitrifying bacteria in flue-cured tobacco soils

    YU Wei; ZHANG Zongjin; GU Yunfu; CHEN Qiang; ZHANG Yingjie; YAN Fangfang
    Panzhihua City Company of Sichuan Tobacco Company, Panzhihua City, P.R. China
    Variation of the denitrifying bacterial communities in the flue-cured tobacco cultivation soils treated with plant growth promoting rhizobacteria (PGPR) plus reduction of chemical fertiliser (RCF) was investigated so as to reveal the microbial...
  5. CORESTA Congress, Kunming, 2018, Smoke Science/Product Technology Groups, ST 25

    Misperception of e-cigarette harm growing among American adults, 2013-2015

    VERRON T.(1); GUO M.(1); VARIGNON B.(1); O'CONNELL G.(2); WALELE T.(2); CAHOURS X.(1)
    (1) SEITA - Imperial Brands plc, Paris, France; (2) Fontem Ventures B.V., Amsterdam, The Netherlands
    Electronic cigarettes (e cigarettes) have been characterised as significantly less harmful than smoked tobacco by an increasing number of public health authorities. However, the proportion of American adults who perceive e cigarettes to be equally or...
  6. CORESTA Congress, Kunming, 2018, Smoke Science/Product Technology Groups, STPOST 43

    In vitro micronucleus (MN) assay using TK6 cells: review of historical positive and negative control data

    STANKOWSKI L.F. Jr(1); HURTADO S.B.(1); WELLS M.M.(1); KWOK V.Y.(1); DOSHI U.(2); COFFA B.G.(2); LEE K.M.(2)
    (1) Charles River Laboratories, Genetic and In Vitro Toxicology, Skokie, IL, U.S.A.; (2) Altria Client Services LLC, Research, Development & Regulatory Affairs, Richmond, VA, U.S.A.
    TK6 human lymphoblastoid cells are widely used in the in vitro MN assay as a part of regulatory genotoxicity testing for pharmaceuticals, but have seen relatively limited use in testing of other test materials, including tobacco-related products. As...
  7. CORESTA Congress, Kunming, 2018, Smoke Science/Product Technology Groups, STPOST 46

    Validation of an in vitro test battery to evaluate smokeless tobacco products

    HURTADO S.B.(1); LALLIER B.(1); TYDRICK C.(1); LIU Z.(2); GUMP J.(2); BISWAS K.(2); KWOK V.Y.(1); STANKOWSKI L.F. Jr(1)
    (1) Charles River Laboratories, Genetic and In Vitro Toxicology, Skokie, IL, U.S.A.; (2) Charles River Laboratories, Analytical Chemistry, Ashland, OH, U.S.A.
    Evaluating potential cytotoxicity and genotoxicity of smokeless tobacco products poses unique challenges for in vitro testing. Each product is a complex mixture of vegetable matter, and may include a packaging component as part of the final product....
  8. CORESTA Congress, Kunming, 2018, Smoke Science/Product Technology Groups, STPOST 52

    Biological test procedure for fresh generated cigarette smoke and e-cigarette aerosols under air-liquid interface (ALI) exposure in 24 and 96 multi-well plates

    WIECZOREK R.(1); TRELLES STICKEN E.(1); WALELE T.(2); CZEKALA L.(3); SIMMS L.(3)
    (1) Reemtsma Cigarettenfabriken GmbH, Imperial Brands plc, Hamburg, Germany; (2) Fontem Ventures, Amsterdam, The Netherlands; (3) Imperial Tobacco Limited, Bristol, U.K.
    Cigarette smoke and e cigarette aerosol contain particulate matter/liquid droplets in the solid phase as well as volatile substances in the gas phase. Both fractions are of potential toxicological relevance and should be considered when assessing...