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  1. Tobacco Science & Technology, 2011, 10, p. 65-69., ISSN.1002-0861

    Development and preliminary application of one-step RT-PCR kit for detection of tobacco CMV

    ZHANG JUN-QI(1); LIU XIANG-BING(2); LUO ZHAO-PENG(1); YANG JUN(1); SONG JI-ZHEN(1); YIN QI-SHENG(1); SONG HAO(2)
    1. Zhengzhou Tobacco Research Institute of CNTC, Zhengzhou 450001, P.R. China; 2. China Tobacco Hunan Industrial Co., Ltd., Changsha 410007, P.R. China
    To detect tobacco CMV quickly and accurately, a pair of specific primers was designed according to the relatively conservative region in the CMV genomic sequence, and enzymes and buffers needed for reverse transcription and PCR were integrated. By...