TSRC, Tob. Sci. Res. Conf., 2018, 72, abstr. 112
Development and verification of a black shank race 0 qPCR assay derived from RAPD marker assay
(1) R.J. Reynolds Tobacco Company, Winston-Salem, NC, USA; (2) North Carolina State University, Raleigh, NC, USA
Historically, Ph gene coupling and repulsion RAPD marker assays (Johnson et al, 2002) have been used to screen for resistant and susceptible tobacco cultivars. Using them as a foundation for our work, DNA fragments from RAPD assays were extracted...