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4 results

  1. Tobacco Science & Technology, 2012, 10, p. 77-80, ISSN.1002-0861

    Adsorption purification of TMV antiserum and detection application

    LUO Zhaopeng(1); WEI Chunyang(1); LI Naihui(2); YANG Jun(1); JIN Dawei(1); JIN Lifeng(1); LI Feng(1); LIN Fucheng(1)
    (1) Zhengzhou Tobacco Research Institute of CNTC, Zhengzhou 450001, P.R. China; (2) Linyi Tobacco Limited Company of Shandong Province, Linyi 276003, Shandong, P.R. China
    For the detection of TMV in tobacco, antiserum was prepared by injecting purified TMV virion into mouse and the titer of antiserum was determined after adsorption purification, an ELISA analysis system was established basing on the in-house TMV...
  2. Tobacco Science & Technology, 2012, 12, p. 72-77, ISSN.1002-0861

    Serological identification of viruses infecting tobacco in major tobacco growing areas of China

    LUO Zhaopeng; YANG Jun; WANG Ran; LI Feng; JIN Lifeng; WEI Chunyang; LIN Fucheng
    Zhengzhou Tobacco Research Institute of CNTC, Zhengzhou 450001, P.R. China
    In order to better understand the occurrence of tobacco virus diseases in China status quo, 409 symptomatic tobacco samples were collected from 13 major tobacco growing areas of China in 2010 and 2011. Tobacco mosaic virus (TMV), cucumber mosaic...
  3. Tobacco Science & Technology, 2012, 5, p. 60-65, 78, ISSN.1002-0861

    Cloning and characterization of a new gene encoding geranylgeranyl pyrophosphate synthase from Nicotiana tabacum

    LI Feng(1); LI Ming(2); JIN Lifeng(1); LUO Zhaopeng(1); YANG Jun(1); LIN Fucheng(*1)
    (1) Zhengzhou Tobacco Research Institute of CNTC, Zhengzhou 450001, P.R. China; (2) Institute of Botany, CAS, Beijing 100093, P.R. China
    A new cDNA sequence encoding GGPPS (designated as Ntggpps3) from tobacco (Nicotiana tabacum) was cloned by RACE techniques and assayed by bioinformatics. The cDNA sequence of Ntggpps3 was of 1251 bp with an open reading frame (ORF) of 1092 bp...
  4. CORESTA Meeting, Agronomy/Phytopathology, 2011, Santiago, PPOST 04

    Molecular variation characterization of the genome 5' terminal region of PVY isolated from tobacco in China

    YANG Jun; LUO Zhao-peng; JIN Li-feng; ZHAI Niu; LI Feng; XU Ya-long; ZHOU Hui-na; LIN Fu-cheng
    Zhengzhou Tobacco Research Institute, Zhegzhou, Henan, P.R. China.
    Tobacco viral diseases, of which most are RNA viruses, are a category of infectious diseases that are distributed widely and occur severely. The natural variation (mutation) of tobacco viruses proceed quickly, and cause considerable difficulty for...