Search Results

95 results

  1. CORESTA Congress, Kunming, 2018, Smoke Science/Product Technology Groups, STPOST 43

    In vitro micronucleus (MN) assay using TK6 cells: review of historical positive and negative control data

    STANKOWSKI L.F. Jr(1); HURTADO S.B.(1); WELLS M.M.(1); KWOK V.Y.(1); DOSHI U.(2); COFFA B.G.(2); LEE K.M.(2)
    (1) Charles River Laboratories, Genetic and In Vitro Toxicology, Skokie, IL, U.S.A.; (2) Altria Client Services LLC, Research, Development & Regulatory Affairs, Richmond, VA, U.S.A.
    TK6 human lymphoblastoid cells are widely used in the in vitro MN assay as a part of regulatory genotoxicity testing for pharmaceuticals, but have seen relatively limited use in testing of other test materials, including tobacco-related products. As an ...
  2. CORESTA Congress, Kunming, 2018, Smoke Science/Product Technology Groups, STPOST 46

    Validation of an in vitro test battery to evaluate smokeless tobacco products

    HURTADO S.B.(1); LALLIER B.(1); TYDRICK C.(1); LIU Z.(2); GUMP J.(2); BISWAS K.(2); KWOK V.Y.(1); STANKOWSKI L.F. Jr(1)
    (1) Charles River Laboratories, Genetic and In Vitro Toxicology, Skokie, IL, U.S.A.; (2) Charles River Laboratories, Analytical Chemistry, Ashland, OH, U.S.A.
    Evaluating potential cytotoxicity and genotoxicity of smokeless tobacco products poses unique challenges for in vitro testing. Each product is a complex mixture of vegetable matter, and may include a packaging component as part of the final product. ...
  3. CORESTA Meeting, Agronomy/Phytopathology, 2017, Santa Cruz do Sul, AP 02

    Reverse logistics - receiving program for empty containers of pesticides from tobacco growers in southern Brazil

    SEHN C.A.
    SindiTabaco - Interstate Tobacco Industry Union, Santa Cruz do Sul, RS, Brazil
    The Brazilian legislation, through Decree 4.074/2002, has determined that users of agrochemicals and related products should return the empty containers and their covers to the commercial establishments where they were purchased. This legislation also ...
  4. CORESTA Meeting, Agronomy/Phytopathology, 2017, Santa Cruz do Sul, AP 22

    Evaluation of soil solarisation as a tool for Integrated Pest Management (IPM) in small tobacco farms in southern Brazil

    BERGER I.J.(1); OLIVEIRA G.H.N.(1); SEHN C.A.(1); SILVA D.J.(1); TEIXEIRA D.V.(1); SARTORI L.(1); SCHMITT M.A.(1); COUGO L.F.(1); FRANCHINI E.(1); CHAVES I.C.P.V.(2); CHAVES L.C.S.V.(2); CARDOZA Y.F.(3); ANDRADE C.C.L.(3); DUARTE V.(3); SANTIN R.C.M.(3)
    (1) SindiTabaco - Interstate Tobacco Industry Union, Santa Cruz do Sul, RS, Brazil; (2) SANTAGRO, Santa Cruz Agrícola Comercial Ltda, Santa Cruz do Sul, RS, Brazil; (3) Agronômica - Phytosanitary Laboratory and Consulting, Porto Alegre, RS, Brazil
    Soil solarisation has a successful history as an environmentally friendly tool for IPM using solar power to control a range of soilborn plant pathogens. Therefore, this technique was evaluated in tobacco production areas in southern Brazil as a ...
  5. CORESTA Meeting, Smoke Science/Product Technology, 2017, Kitzbühel, STPOST 44

    Validation and comparison of the neutral red uptake assay in BALB/c 3T3 and CHO-WBL cells

    HURTADO S.B; CALLUPE J.; MARTINEZ J.L.; SOOMER-JAMES J.; GROMER K.D.; KWOK V.Y.; FARABAUGH C.S.; STANKOWSKI L.F. Jr.
    Charles River Laboratories - Skokie, Skokie, IL, U.S.A.
    The neutral red uptake (NRU) assay is used to evaluate the cytotoxicity of a variety of chemicals, cigarette smoke fractions, e-liquids, medical devices, etc. in vitro. Charles River Laboratories-Skokie previously validated the NRU assay in BALB/c 3T3 ...
  6. CORESTA Meeting, Smoke Science/Product Technology, 2017, Kitzbühel, STPOST 46

    Validation and comparison of the in vitro micronucleus assay in TK6 and CHO-WBL cells

    WELLS M.; LORENZ M.; SOOMER-JAMES J.; KAYE S.L.; GROMER K.D.; BHATTACHARYA R.; OCO K.R.; KWOK V.Y.; FARABAUGH C.S.; STANKOWSKI L.F. Jr.
    Charles River Laboratories - Skokie, Skokie, IL, U.S.A.
    The in vitro micronucleus (MN) assay is used to evaluate the potential clastogenicity and aneugenicity of a variety of chemicals and agents in vitro. Charles River Laboratories-Skokie previously validated the in vitro MN assay in TK6 cells, according to ...
  7. 47th TWC, Tob. Work. Conf., 2016, abstr. 29

    Working towards an increased understanding of the genetics of soil-borne disease resistance in tobacco

    DRAKE K.(1); LEWIS R.S.(1); BAKAHER N.(2); GOEPFERT S.(2); PHILIPPON B.(2); MARK R.(2); PETERSON P.D.(3)
    (1) North Carolina State University, Raleigh NC USA; (2) Philip Morris International, Neuchatel, Switzerland; (3) Clemson University, Florence SC USA
    Black shank and bacterial wilt, caused by Phytophthora nicotianae and Ralstonia solanacearum, respectively, are the most important diseases affecting tobacco production in the United States. An increased understanding of the genetic systems controlling ...