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3 results

  1. CORESTA Congress, Sapporo, 2012, Agronomy/Phytopathology Groups, APPOST 09

    Cloning and bioinformatic analysis of orange protein (OR) gene from Nicotiana tabacum

    LEI Bo(1); LU Kun(2); DING Fu-zhang(1); LIU Xiao-rong(2); HU Zhong-yi(1); PAN Wen-jie(1)
    (1) Guizhou Tobacco Science Research Institute, Guiyang, Guizhou, P.R. China; (2) College of Agronomy and Biothehnology of Southwest University, Chongqing, P.R. China
    Previous microarray results indicated that the orange protein (OR) gene in the carotenoid biosynthesis pathway showed significant differential expression in leaves between moderate and light aroma tobacco areas. In order to obtain the full-length sequence ...
  2. Tobacco Science & Technology, 2012, 4, p. 60-66, ISSN.1002-0861

    Cloning and bioinformatics analysis of orange protein (OR) gene from Nicotiana tabacum

    LEI Bo(1); LU Kun(2); DING Fuzhang(1); LIU Xiaorong(2); HU Zhongyi(1); PAN Wenjie(1)
    (1) Guizhou Tobacco Research Institute, Guiyang 550081, P.R. China; (2) College of Agronomy and Biotechnology of Southwest University, Chongqing 400715, P.R. China
    In order to obtain the full-length sequence of NtOR and reveal its biological function, the full-length cDNA and genomic DNA sequences of NtOR were cloned from cv. K326 using homologous cloning method, and logged in GenBank (accession numbers JN379458 and ...
  3. CORESTA Meeting, Agronomy/Phytopathology, 2011, Santiago, APOST 07

    Comparative transcriptome of tobacco leaves in typical ecological areas of Guizhou

    LEI Bo(1); PAN Wenjie(1); DING Fuzhang(1); ZHANG Kai(2); LI Jinxin(3); LU Ku(2)
    (1) Guizhou Tobacco Research Institute, Guiyang, P.R China; (2) College of Agronomy and Biotechnology, Southwest University, Chongqing, P.R China; (3) Guizhou Provincial Tobacco Company, Guiyang, P.R China
    Flue-cured tobacco (Nicotiana tabacum) is a sensitive crop to ecological environment, and distinctive tobacco has always formed under special ecological conditions. In order to investigate the molecular mechanism of formation of medium and light aroma ...