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68 results

  1. CORESTA Congress, Kunming, 2018, Smoke Science/Product Technology Groups, STPOST 43

    In vitro micronucleus (MN) assay using TK6 cells: review of historical positive and negative control data

    STANKOWSKI L.F. Jr(1); HURTADO S.B.(1); WELLS M.M.(1); KWOK V.Y.(1); DOSHI U.(2); COFFA B.G.(2); LEE K.M.(2)
    (1) Charles River Laboratories, Genetic and In Vitro Toxicology, Skokie, IL, U.S.A.; (2) Altria Client Services LLC, Research, Development & Regulatory Affairs, Richmond, VA, U.S.A.
    TK6 human lymphoblastoid cells are widely used in the in vitro MN assay as a part of regulatory genotoxicity testing for pharmaceuticals, but have seen relatively limited use in testing of other test materials, including tobacco-related products. As an ...
  2. CORESTA Congress, Kunming, 2018, Smoke Science/Product Technology Groups, STPOST 46

    Validation of an in vitro test battery to evaluate smokeless tobacco products

    HURTADO S.B.(1); LALLIER B.(1); TYDRICK C.(1); LIU Z.(2); GUMP J.(2); BISWAS K.(2); KWOK V.Y.(1); STANKOWSKI L.F. Jr(1)
    (1) Charles River Laboratories, Genetic and In Vitro Toxicology, Skokie, IL, U.S.A.; (2) Charles River Laboratories, Analytical Chemistry, Ashland, OH, U.S.A.
    Evaluating potential cytotoxicity and genotoxicity of smokeless tobacco products poses unique challenges for in vitro testing. Each product is a complex mixture of vegetable matter, and may include a packaging component as part of the final product. ...
  3. CORESTA Meeting, Smoke Science/Product Technology, 2017, Kitzbühel, STPOST 44

    Validation and comparison of the neutral red uptake assay in BALB/c 3T3 and CHO-WBL cells

    HURTADO S.B; CALLUPE J.; MARTINEZ J.L.; SOOMER-JAMES J.; GROMER K.D.; KWOK V.Y.; FARABAUGH C.S.; STANKOWSKI L.F. Jr.
    Charles River Laboratories - Skokie, Skokie, IL, U.S.A.
    The neutral red uptake (NRU) assay is used to evaluate the cytotoxicity of a variety of chemicals, cigarette smoke fractions, e-liquids, medical devices, etc. in vitro. Charles River Laboratories-Skokie previously validated the NRU assay in BALB/c 3T3 ...
  4. CORESTA Meeting, Smoke Science/Product Technology, 2017, Kitzbühel, STPOST 46

    Validation and comparison of the in vitro micronucleus assay in TK6 and CHO-WBL cells

    WELLS M.; LORENZ M.; SOOMER-JAMES J.; KAYE S.L.; GROMER K.D.; BHATTACHARYA R.; OCO K.R.; KWOK V.Y.; FARABAUGH C.S.; STANKOWSKI L.F. Jr.
    Charles River Laboratories - Skokie, Skokie, IL, U.S.A.
    The in vitro micronucleus (MN) assay is used to evaluate the potential clastogenicity and aneugenicity of a variety of chemicals and agents in vitro. Charles River Laboratories-Skokie previously validated the in vitro MN assay in TK6 cells, according to ...
  5. 47th TWC, Tob. Work. Conf., 2016, abstr. 29

    Working towards an increased understanding of the genetics of soil-borne disease resistance in tobacco

    DRAKE K.(1); LEWIS R.S.(1); BAKAHER N.(2); GOEPFERT S.(2); PHILIPPON B.(2); MARK R.(2); PETERSON P.D.(3)
    (1) North Carolina State University, Raleigh NC USA; (2) Philip Morris International, Neuchatel, Switzerland; (3) Clemson University, Florence SC USA
    Black shank and bacterial wilt, caused by Phytophthora nicotianae and Ralstonia solanacearum, respectively, are the most important diseases affecting tobacco production in the United States. An increased understanding of the genetic systems controlling ...
  6. CORESTA Congress, Berlin, 2016, Agronomy/Phytopathology Groups, AP 41

    Investigating the genetics of polygenic soil-borne disease resistance in tobacco

    LEWIS R.S.(1); DRAKE-STOWE K.(1); BAKAHER N.(2); GOEPFERT S.(2); PHILIPPON B.(2); MARK R.(2); PETERSON P.(3)
    (1) North Carolina State University, Raleigh, NC, U.S.A.; (2) Philip Morris International R&D, Philip Morris Products S.A, (part of Philip Morris International group of companies), Neuchâtel, Switzerland; (3) Clemson University, Florence, NC, U.S.A.
    Bacterial wilt and black shank, caused by Ralstonia solanacearum and Phytophthora nicotianae, respectively, are the most important diseases affecting tobacco production in the United States. An increased understanding of the genetics controlling ...
  7. 46th TWC, Tob. Work. Conf., 2014, abstr. 29

    Variable rate irrigation in South Carolina flue-cured tobacco

    HENDERSON R.D.; GOODEN D.T.; FORTNUM B.A.
    Clemson University, Pee Dee Research and Education Center, 2200 Pocket Road, Florence, SC 29506-9706, USA
    Extended periods of drought are not uncommon in the flue-cured tobacco production area of South Carolina during the growing season. Timely application of sufficient volumes of water during critical phases of crop development can have a dramatic impact on ...
  8. 46th TWC, Tob. Work. Conf., 2014, abstr. 42

    Solar assisted curing in flue-cured tobacco

    HENDERSON R.D.; REED T.D.; GOODEN D.T.; FORTNUM B.A.
    Clemson University, Pee Dee Research and Education Center, 2200 Pocket Road, Florence, SC 29506-9706, USA
    The objective of this study was to assess the relative efficiency of a prototype solar flue-curing barn that utilizes heat collected in a plenum around the outer metal wall of the barn to preheat the air going into the barn and burner. Part of this ...