Search Results

13 results

  1. CORESTA Meeting, Smoke Science/Product Technology, 2013, Seville, ST 41

    Activation of transcription factors in human bronchial epithelial cells exposed to aqueous extracts of mainstream cigarette smoke in vitro

    SEKINE T.; HIRATA T.; MINE T.; FUKANO Y.
    Japan Tobacco Inc., Product Science Division, R&D Group, Yokohama, Japan
    Transcription factors are recognised as important components of signalling cascades controlling all types of normal cellular processes as well as response to external stimulus. Activation of transcription factors can alter the expression of specific genes ...
  2. CORESTA Congress, Sapporo, 2012, Smoke Science/Product Technology Groups, SSPTPOST 04

    Report on biological researches using a heated cigarette. Part 4: In vitro oxidative stress assays

    SUZUKI H.; NISHIJIMA Y.; FUKANO Y.
    Japan Tobacco Inc., R&D Group, Product Science Division, Yokohama, Kanagawa, Japan
    Mainstream cigarette smoke (MCS) is a mixture of several thousand constituents and is reported to include many oxidants, such as reactive oxygen species (ROS). ROS induced oxidative damage at molecular and cellular levels. At the 2010 CORESTA Congress ...
  3. CORESTA Meeting, Smoke Science/Product Technology, 2011, Graz, ST 33

    An induction process for 8-hydroxy-2'deoxyguanosine (8-OHdG) induced by aqueous extracts of mainstream cigarette smoke in cultured cells

    NISHIJIMA Y.; FUKANO Y.
    Product Science Division, Japan Tobacco Inc., Yokohama, Kanagawa, Japan
    Oxidative stress is a state of imbalance between the reactive oxygen species (ROS) and the ability of the organism to defend against them, leading to progressive oxidative damage.8-hydroxy-2'deoxyguanosine (8-OHdG) is one of the markers for oxidative ...
  4. CORESTA Congress, Edinburgh, 2010, SSPT 41

    The role of of cigarette smoke constituents in oxidative modification by aqueous extracts of mainstream cigarette smoke (part I) 8-OhdG

    NISHIJIMA Y.; FUKANO Y.
    Japan Tobacco Inc., R&D Group, Product Science Division, Yokohama, Kanagawa, Japan
    Oxidative stress is a state of imbalance between reactive oxygen species (ROS), and the ability of the organism to defend against them, leading to progressive oxidative damage. ROS causes oxidation of proteins, DNA and lipids, which may cause direct ...
  5. CORESTA Congress, Edinburgh, 2010, SSPT 42

    The role of cigarette smoke constituents in oxidative modification by aqueous extracts of mainstream cigarette smoke (part II) LDL lipid peroxidation.

    SUZUKI H.; FUKANO Y.
    Japan Tobacco Inc., R&D Group, Product Science Division, Yokohama, Kanagawa, Japan
    Oxidative stress is a state of imbalance between reactive oxygen species (ROS), and the ability of the organism to defend against them, leading to progressive oxidative damage. ROS causes oxidation of proteins, DNA and lipids, which may cause direct ...
  6. CORESTA Meeting, Smoke Science/Product Technology, Jeju, 2007, SSPT 43

    Detection of cytotoxicity in the water-insoluble fraction of cigarette smoke gas vapor phase using a whole smoke exposure system

    NISHINO T.; NARA H.; FUKANO Y.
    Japan Tobacco Inc., Tobacco Science Research Lab., Yokohama, Kanagawa, Japan.
    When biological evaluations of cigarette smoke are conducted, the particulate phase (PP) and gas vapor phase (GVP) of mainstream smoke are collected separately and exposed to cultured cells. GVP is generally collected in phosphate buffer saline (PBS) by ...
  7. CORESTA Congress, Paris, 2006, SS 31

    Differences in the generation of 8-hydroxyguanine by cigarette smoke in calf-thymus DNA and in A549 cells

    MIURA N.; FUKANO Y.; NISHINO T.
    Japan Tobacco Inc., Tobacco Science Research Lab., Yokohama, Kanagawa, Japan.
    In this report we investigated the generation of 8-hydroxyguanine by cigarette smoke using calf-thymus DNA and A549 cells. At first, we exposed the calf-thymus DNA to cigarette smoke bubbled PBS and measured the amount of 8-OhdG, which is the hydrolysate ...