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146 results

  1. CORESTA Congress, Kunming, 2018, Agronomy/Phytopathology Groups, AP 49

    The influence of Nicotiana alata-derived introgression on plant malformations of tobacco breeding lines resistant to tomato spotted wilt virus

    KORBECKA-GLINKA G.(1); TROJAK-GOLUCH A.(1); DOROSZEWSKA T.(1); GOEPFERT S.(2)
    (1) Institute of Soil Science and Plant Cultivation - State Research Institute, Pulawy, Poland; (2) Philip Morris Products S.A. (part of Philip Morris International group of companies), PMI R&D, Neuchâtel, Switzerland
    Tomato spotted wilt virus (TSWV) is one of the most destructive viruses for tobacco cultivation. Cultivar “Polalta” carries a resistance gene introgressed from a wild species, Nicotiana alata, but the use of this cultivar in breeding is hampered by...
  2. CORESTA Congress, Kunming, 2018, Agronomy/Phytopathology Groups, APPOST 16

    Monitoring of agricultural drought in tobacco growing areas in Poland in 2009-2017

    BERBEĆ T.; DOROSZEWSKI A.; BERBEĆ A.
    Institute of Soil Science and Plant Cultivation - State Research Institute, Pulawy, Poland
    Occurrence of unfavourable weather conditions (drought) causes significant losses in crop yields in the world. McKeown et al. [2006], in their work on long-term climate patterns related to yields, stated that by 2030 the periodic occurrence of...
  3. CORESTA Congress, Kunming, 2018, Agronomy/Phytopathology Groups, APPOST 17

    Comparison of the degree of malformations in different breeding materials carrying Nicotiana alata-introgression conferring resistance to tomato spotted wilt virus

    TROJAK-GOLUCH A.(1); KORBECKA-GLINKA G.(1); DOROSZEWSKA T.(1); GOEPFERT S.(2)
    Institute of Soil Science and Plant Cultivation - State Research Institute, Pulawy, Poland
    Tomato spotted wilt virus (TSWV) has been an important problem in tobacco cultivated in a moderate climate zone. The transfer of RTSW-al (TSWV resistance gene) from Nicotiana alata to the N. tabacum genome and the development of Virginia-type...
  4. CORESTA Congress, Kunming, 2018, Agronomy/Phytopathology Groups, APPOST 26

    Nicotiana species as sources of cytoplasmic male sterility in tobacco breeding

    BERBEĆ A.; BERBEĆ T.
    Institute of Soil Science and Plant Cultivation - State Research Institute, Pulawy, Poland
    The majority of cytoplasmically male sterile (cms) forms in tobacco, Nicotiana tabacum, have been produced by moving the whole nuclear genome from one species and re-installing it in the cytoplasmic milieu of another species thereby disrupting the...
  5. CORESTA Congress, Kunming, 2018, Smoke Science/Product Technology Groups, STPOST 43

    In vitro micronucleus (MN) assay using TK6 cells: review of historical positive and negative control data

    STANKOWSKI L.F. Jr(1); HURTADO S.B.(1); WELLS M.M.(1); KWOK V.Y.(1); DOSHI U.(2); COFFA B.G.(2); LEE K.M.(2)
    (1) Charles River Laboratories, Genetic and In Vitro Toxicology, Skokie, IL, U.S.A.; (2) Altria Client Services LLC, Research, Development & Regulatory Affairs, Richmond, VA, U.S.A.
    TK6 human lymphoblastoid cells are widely used in the in vitro MN assay as a part of regulatory genotoxicity testing for pharmaceuticals, but have seen relatively limited use in testing of other test materials, including tobacco-related products. As...
  6. CORESTA Congress, Kunming, 2018, Smoke Science/Product Technology Groups, STPOST 46

    Validation of an in vitro test battery to evaluate smokeless tobacco products

    HURTADO S.B.(1); LALLIER B.(1); TYDRICK C.(1); LIU Z.(2); GUMP J.(2); BISWAS K.(2); KWOK V.Y.(1); STANKOWSKI L.F. Jr(1)
    (1) Charles River Laboratories, Genetic and In Vitro Toxicology, Skokie, IL, U.S.A.; (2) Charles River Laboratories, Analytical Chemistry, Ashland, OH, U.S.A.
    Evaluating potential cytotoxicity and genotoxicity of smokeless tobacco products poses unique challenges for in vitro testing. Each product is a complex mixture of vegetable matter, and may include a packaging component as part of the final product....
  7. CORESTA Meeting, Smoke Science/Product Technology, 2017, Kitzbühel, STPOST 44

    Validation and comparison of the neutral red uptake assay in BALB/c 3T3 and CHO-WBL cells

    HURTADO S.B; CALLUPE J.; MARTINEZ J.L.; SOOMER-JAMES J.; GROMER K.D.; KWOK V.Y.; FARABAUGH C.S.; STANKOWSKI L.F. Jr.
    Charles River Laboratories - Skokie, Skokie, IL, U.S.A.
    The neutral red uptake (NRU) assay is used to evaluate the cytotoxicity of a variety of chemicals, cigarette smoke fractions, e-liquids, medical devices, etc. in vitro. Charles River Laboratories-Skokie previously validated the NRU assay in BALB/c...
  8. CORESTA Meeting, Smoke Science/Product Technology, 2017, Kitzbühel, STPOST 46

    Validation and comparison of the in vitro micronucleus assay in TK6 and CHO-WBL cells

    WELLS M.; LORENZ M.; SOOMER-JAMES J.; KAYE S.L.; GROMER K.D.; BHATTACHARYA R.; OCO K.R.; KWOK V.Y.; FARABAUGH C.S.; STANKOWSKI L.F. Jr.
    Charles River Laboratories - Skokie, Skokie, IL, U.S.A.
    The in vitro micronucleus (MN) assay is used to evaluate the potential clastogenicity and aneugenicity of a variety of chemicals and agents in vitro. Charles River Laboratories-Skokie previously validated the in vitro MN assay in TK6 cells, according...
  9. CORESTA Congress, Berlin, 2016, Agronomy/Phytopathology Groups, APPOST 08

    Agronomic performance of cytoplasmic male sterile forms of flue-cured tobacco

    CZUBACKA A.; DEPTA A.; DOROSZEWSKA T.
    Institute of Soil Science and Plant Cultivation - State Research Institute, Pulawy, Poland
    Cytoplasmic male sterility (cms) in many crops is the basis for the production of commercial F1 hybrids. This is obtained by the combination of the nucleus of cultivated species with the cytoplasm of a wild species belonging to the same genus. The...
  10. CORESTA Congress, Berlin, 2016, Agronomy/Phytopathology Groups, APPOST 23

    Influence of PVY infection on chemical composition of tobacco

    PRZYBYS M.; CZUBACKA A.
    Institute of Soil Science and Plant Cultivation - State Research Institute, Pulawy, Poland
    Potato virus Y (PVY) is the type member of the genus Potyvirus in family Potyviridae. PVY has a worldwide distribution and is one of the most economically important viruses in tobacco. PVY causes limitation of assimilation, gas exchange, inhibition...