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9 results

  1. CORESTA Congress, Kunming, 2018, Smoke Science/Product Technology Groups, STPOST 43

    In vitro micronucleus (MN) assay using TK6 cells: review of historical positive and negative control data

    STANKOWSKI L.F. Jr(1); HURTADO S.B.(1); WELLS M.M.(1); KWOK V.Y.(1); DOSHI U.(2); COFFA B.G.(2); LEE K.M.(2)
    (1) Charles River Laboratories, Genetic and In Vitro Toxicology, Skokie, IL, U.S.A.; (2) Altria Client Services LLC, Research, Development & Regulatory Affairs, Richmond, VA, U.S.A.
    TK6 human lymphoblastoid cells are widely used in the in vitro MN assay as a part of regulatory genotoxicity testing for pharmaceuticals, but have seen relatively limited use in testing of other test materials, including tobacco-related products. As...
  2. CORESTA Congress, Kunming, 2018, Smoke Science/Product Technology Groups, STPOST 46

    Validation of an in vitro test battery to evaluate smokeless tobacco products

    HURTADO S.B.(1); LALLIER B.(1); TYDRICK C.(1); LIU Z.(2); GUMP J.(2); BISWAS K.(2); KWOK V.Y.(1); STANKOWSKI L.F. Jr(1)
    (1) Charles River Laboratories, Genetic and In Vitro Toxicology, Skokie, IL, U.S.A.; (2) Charles River Laboratories, Analytical Chemistry, Ashland, OH, U.S.A.
    Evaluating potential cytotoxicity and genotoxicity of smokeless tobacco products poses unique challenges for in vitro testing. Each product is a complex mixture of vegetable matter, and may include a packaging component as part of the final product....
  3. CORESTA Meeting, Smoke Science/Product Technology, 2017, Kitzbühel, STPOST 44

    Validation and comparison of the neutral red uptake assay in BALB/c 3T3 and CHO-WBL cells

    HURTADO S.B; CALLUPE J.; MARTINEZ J.L.; SOOMER-JAMES J.; GROMER K.D.; KWOK V.Y.; FARABAUGH C.S.; STANKOWSKI L.F. Jr.
    Charles River Laboratories - Skokie, Skokie, IL, U.S.A.
    The neutral red uptake (NRU) assay is used to evaluate the cytotoxicity of a variety of chemicals, cigarette smoke fractions, e-liquids, medical devices, etc. in vitro. Charles River Laboratories-Skokie previously validated the NRU assay in BALB/c...
  4. CORESTA Meeting, Smoke Science/Product Technology, 2017, Kitzbühel, STPOST 46

    Validation and comparison of the in vitro micronucleus assay in TK6 and CHO-WBL cells

    WELLS M.; LORENZ M.; SOOMER-JAMES J.; KAYE S.L.; GROMER K.D.; BHATTACHARYA R.; OCO K.R.; KWOK V.Y.; FARABAUGH C.S.; STANKOWSKI L.F. Jr.
    Charles River Laboratories - Skokie, Skokie, IL, U.S.A.
    The in vitro micronucleus (MN) assay is used to evaluate the potential clastogenicity and aneugenicity of a variety of chemicals and agents in vitro. Charles River Laboratories-Skokie previously validated the in vitro MN assay in TK6 cells, according...
  5. CORESTA Meeting, Agronomy/Phytopathology, 2015, Izmir, Turkey, IG 01 ; CORESTA Meeting, Smoke Science/Product Technology, 2015, Jeju, IG 01

    Association mapping in a collection of tobacco reference cultivars. Step One: Variability of smoke constituents

    JULIO E.(1); VERRON T.(2); CAHOURS X.(2); DORLHAC de BORNE F.(1); COLARD S.(2)
    (1) SEITA, Imperial Tobacco Group, Bergerac, France; (2) SEITA, Imperial Tobacco Group, Fleury-les-Aubrais, France
    Recent developments of sequencing technologies and computational methods have given the opportunity to detect natural variation underlying complex traits in crops. For this purpose, association mapping can be used to identify the link between tobacco...
  6. CORESTA Congress, Quebec, 2014, Agronomy/Phytopathology Groups, APPOST 03

    Agrobacterium T-DNA in Nicotiana tomentosiformis and other Nicotiana species

    CHEN K.(1); DORLHAC DE BORNE F.(2); SZEGEDI E.(3); OTTEN L.(1)
    (1) Department of Molecular Mechanisms of Phenotypic Plasticity, Institut de Biologie Moléculaire des Plantes du C.N.R.S., Strasbourg, France; (2) National Agricultural Research and Innovation Centre (NARIC), Research Institute for Viticulture and Enology, Experimental Station of Kecskemét, Hungary; (3) Imperial Tobacco Group, Institut du Tabac, Bergerac, France
    Agrobacterium rhizogenes induces growth of aberrant roots with bacterial T-DNA; some roots can spontaneously regenerate into plants carrying T-DNA fragments. T-DNAs contain genes that are expressed in plants which normally lead to root growth and to...
  7. CORESTA Congress, Quebec, 2014, Smoke Science/Product Technology Groups, ST 01

    Stalk position effect on harmful and potentially harmful cigarette smoke constituents in Burley tobacco: a case study

    VERRIER J-L.(1); DESTRUHAUT S.(2); COLARD S.(3)
    (1) Imperial Tobacco Group, Institut du Tabac, Bergerac, France; (2) Imperial Tobacco Group, SEITA, Fleury-les-Aubrais, France; (3) Imperial Tobacco Ltd, Bristol, U.K.
    Cured leaf chemistry varies sharply according to stalk positions and variations of smoke yields of harmful and potentially harmful constituents (HPHCs) are also expected. To quantitatively assess the effects on HPHC yields, Burley leaves of the...
  8. TSRC, Tob. Sci. Res. Conf., 2011, 65, abstr. 20

    An in vitro comparative study of [14c]-eugenol and [14c]-methyleugenol activation and detoxification kinetics in human, mouse, and rat liver fractions.

    MINET E.(1); GENTILE D.(2); MEREDITH C.(1); MASSEY E.D.(1)
    (1) British American Tobacco, Group R&D, Southampton, UK; (2) Charles River Laboratories, Edinburgh, UK
    Introduction - Eugenol is a natural alkenylbenzene compound used in a variety of consumer products including Kretek cigarettes. There is limited evidence for the carcinogenicity of eugenol to experimental animals. However, in vitro tests for the...
  9. CORESTA Meeting, Smoke Science/Product Technology, Stratford-upon-Avon, 2005, SSPTPOST 09

    Predictivity and variability of in vitro bioassays routinely used to estimate the toxicity of cigarette smoke

    PROUDLOCK R.; LAPIERRE J.; MORIN A.
    Charles River Laboratories, Montreal, Imperial Tobacco Canada Limited, Montreal, Canada.
    An amendment to the Tobacco Act is currently being introduced in Canada. This will require all brands of cigarettes sold in this country to be subjected to a set of three in vitro assays on an annual basis. The purpose of the amendment is to monitor...