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111 results

  1. CORESTA Congress, Kunming, 2018, Smoke Science/Product Technology Groups, STPOST 43

    In vitro micronucleus (MN) assay using TK6 cells: review of historical positive and negative control data

    STANKOWSKI L.F. Jr(1); HURTADO S.B.(1); WELLS M.M.(1); KWOK V.Y.(1); DOSHI U.(2); COFFA B.G.(2); LEE K.M.(2)
    (1) Charles River Laboratories, Genetic and In Vitro Toxicology, Skokie, IL, U.S.A.; (2) Altria Client Services LLC, Research, Development & Regulatory Affairs, Richmond, VA, U.S.A.
    TK6 human lymphoblastoid cells are widely used in the in vitro MN assay as a part of regulatory genotoxicity testing for pharmaceuticals, but have seen relatively limited use in testing of other test materials, including tobacco-related products. As an ...
  2. CORESTA Congress, Kunming, 2018, Smoke Science/Product Technology Groups, STPOST 46

    Validation of an in vitro test battery to evaluate smokeless tobacco products

    HURTADO S.B.(1); LALLIER B.(1); TYDRICK C.(1); LIU Z.(2); GUMP J.(2); BISWAS K.(2); KWOK V.Y.(1); STANKOWSKI L.F. Jr(1)
    (1) Charles River Laboratories, Genetic and In Vitro Toxicology, Skokie, IL, U.S.A.; (2) Charles River Laboratories, Analytical Chemistry, Ashland, OH, U.S.A.
    Evaluating potential cytotoxicity and genotoxicity of smokeless tobacco products poses unique challenges for in vitro testing. Each product is a complex mixture of vegetable matter, and may include a packaging component as part of the final product. ...
  3. CORESTA Meeting, Smoke Science/Product Technology, 2017, Kitzbühel, STPOST 44

    Validation and comparison of the neutral red uptake assay in BALB/c 3T3 and CHO-WBL cells

    HURTADO S.B; CALLUPE J.; MARTINEZ J.L.; SOOMER-JAMES J.; GROMER K.D.; KWOK V.Y.; FARABAUGH C.S.; STANKOWSKI L.F. Jr.
    Charles River Laboratories - Skokie, Skokie, IL, U.S.A.
    The neutral red uptake (NRU) assay is used to evaluate the cytotoxicity of a variety of chemicals, cigarette smoke fractions, e-liquids, medical devices, etc. in vitro. Charles River Laboratories-Skokie previously validated the NRU assay in BALB/c 3T3 ...
  4. CORESTA Meeting, Smoke Science/Product Technology, 2017, Kitzbühel, STPOST 46

    Validation and comparison of the in vitro micronucleus assay in TK6 and CHO-WBL cells

    WELLS M.; LORENZ M.; SOOMER-JAMES J.; KAYE S.L.; GROMER K.D.; BHATTACHARYA R.; OCO K.R.; KWOK V.Y.; FARABAUGH C.S.; STANKOWSKI L.F. Jr.
    Charles River Laboratories - Skokie, Skokie, IL, U.S.A.
    The in vitro micronucleus (MN) assay is used to evaluate the potential clastogenicity and aneugenicity of a variety of chemicals and agents in vitro. Charles River Laboratories-Skokie previously validated the in vitro MN assay in TK6 cells, according to ...
  5. CORESTA Meeting, Agronomy/Phytopathology, 2013, Brufa di Torgiano, APPOST 01

    Performance of fire-cured tobacco with compost from urban sources

    COZZOLINO E.; LEONE V.; LOMBARDI P.
    Consiglio per la Ricerca e la Sperimentazione in Agricoltura - Unità di Ricerca per le Colture Alternative al Tabacco (CRA-CAT), Scafati (SA), Italy
    A two-year trial of Kentucky fire-cured tobacco fertilisation with compost from urban organic waste was conducted with three cultivars (Foiano, Riccio beneventano and SKL) on two farms in the province of Benevento (Italy). A commercial compost, produced ...
  6. CORESTA Meeting, Agronomy/Phytopathology, 2013, Brufa di Torgiano, APPOST 04

    Influence of plant density and leaf priming collection on seed and seed oil yield in tobacco

    BUCCIARELLI S.(1); CIVITELLA D.(1); FECONDO G.(1); GHIANNI G.(1); SORRENTINO C.(2); DEL PIANO L.(2); RAIMO F.(2); STANISCI V.(3)
    (1) Consorzio per la Divulgazione e la Sperimentazione delle Tecniche Irrigue (CO.T.IR), Vasto (CH), Italy; (2) Consiglio per la Ricerca e la Sperimentazione in Agricoltura - Unità di Ricerca per le Colture Alternative al Tabacco (CRA-CAT), Scafati (SA), Italy; (3) ECOFOX, Vasto (CH), Italy
    In the EU the Common Agricultural Policy (CAP) reform of 2004 has established a gradual reduction in incentives for the production of tobacco. In Italy there was a reduction of about 30% of the area under tobacco cultivation from 2000 to 2010. Value-added ...
  7. CORESTA Meeting, Agronomy/Phytopathology, 2013, Brufa di Torgiano, APPOST 05

    New cultivars for the Italian Burley industry

    COZZOLINO E.; LEONE V.; LOMBARDI P.
    Consiglio per la Ricerca e la Sperimentazione in Agricoltura - Unità di Ricerca per le Colture Alternative al Tabacco (CRA-CAT), Scafati (SA), Italy
    Lack of information about cultivar adaptation to specific areas is a hindrance to the progress of the tobacco industry. To provide such information for Italian Burley areas, an on-farm variety trial is underway at Vitulazio, in the province of Caserta, ...
  8. CORESTA Meeting, Agronomy/Phytopathology, 2013, Brufa di Torgiano, APPOST 08

    SSR analysis of genetic polymorphism in a Nicotiana Germplasm Collection

    DEL PIANO L.; DI DATO F.; ABET M.; RAIMO F.; SORRENTINO C.; SICIGNANO M.; ENOTRIO T.
    Consiglio per la Ricerca e la Sperimentazione in Agricoltura - Unità di Ricerca per le Colture Alternative al Tabacco (CRA-CAT), Scafati (SA), Italy
    Tobacco (Nicotiana tabacum L.) is an important agricultural crop plant that plays a significant role in the economies of many countries. Tobacco breeding aims to develop varieties with wide adaptability, higher potential and suitable chemical constituents ...
  9. TSRC, Tob. Sci. Res. Conf., 2011, 65, abstr. 20

    An in vitro comparative study of [14c]-eugenol and [14c]-methyleugenol activation and detoxification kinetics in human, mouse, and rat liver fractions.

    MINET E.(1); GENTILE D.(2); MEREDITH C.(1); MASSEY E.D.(1)
    (1) British American Tobacco, Group R&D, Southampton, UK; (2) Charles River Laboratories, Edinburgh, UK
    Introduction- Eugenol is a natural alkenylbenzene compound used in a variety of consumer products including Kretek cigarettes. There is limited evidence for the carcinogenicity of eugenol to experimental animals. However, in vitro tests for the genotoxic ...
  10. CORESTA Congress, Edinburgh, 2010, APPOST 01

    Effect of the degree of leaf ripeness at harvest on the yield, quality and chemical characteristics of Oriental (sun-cured) tobacco

    KARAIVAZOGLOU N.A.; PAPAKOSTA D.K.; DIVANIDIS S.
    Aristotle University of Thessaloniki, Greece; Tobacco Institute of Greece, Chemistry Dept., Drama, Greece
    Harvest date is one of the most important crop management practices affecting tobacco yield, quality and usability after curing. In a two-year field experiment, investigations were made into the effect of the degree of leaf ripeness at harvest on several ...