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3 results

  1. TSRC, Tob. Sci. Res. Conf., 2012, 66, abstr. 13

    Accumulation of nicotine and nornicotine enantiomers in leaf of nicotine demethylases mutants during growth and curing

    CAI B.(1); JACK A.M.(1); LEWIS R.S.(2); DEWEY R.E.(2); JI H.(1); BUSH L.P.(1)
    (1) University of Kentucky, Lexington, KY, USA; (2) North Carolina State University, Raleigh, NC, USA
    Nicotine metabolism in tobacco plants is mostly through N-demethylation, resulting in nornicotine. There are three functional nicotine demethylases CYP82E4 (E4), CYP82E5v2 (E5) and CYP82E10 (E10) in tobacco. In this study, we investigated the...
  2. 44th TWC, Tob. Work. Conf., 2010, abstr. 10

    Development of CAPS and dCAPS markers for CYP82E4 and CYP82E5v2 gene mutants reducing nicotine to nornicotine conversion in tobacco

    LI D.; LEWIS R.S.; JACK A.M.; DEWEY R.E.
    University of Kentucky, Dept. of Plant & Soil Sciences, Lexington, KY, USA
    Nornicotine accumulation in Burley tobacco is of concern because nornicotine is the precursor of the predominant Burley tobacco-specific nitrosamines (TSNAs), N'-nitrosonornicotine (NNN). However, screening of Burley tobacco is problematic because...
  3. CORESTA Congress, Paris, 2006, IG 02

    Functional characterization of the nicotine N-demethylase gene of tobacco

    DEWEY R.E.; SIMINSZKY B.; BOWEN S.W.; GAVILANO L.; WAREK U.; HAYES A.
    North Carolina State University, Dept. of Crop Science, Raleigh, NC, USA; University of Kentucky, Dept. of Plant and Soil Sci., Lexington, KY, USA; Philip Morris USA, Richmond, VA, USA
    In tobacco, nicotine is metabolized to nornicotine via the activity of the nicotine N -demethylase enzyme. During the curing and processing of the leaf, nornicotine serves are the direct precursor in the synthesis of N -nitrosonornicotine (NNN), a...