1. TSRC, Tob. Sci. Res. Conf., 2018, 72, abstr. 066

    Distinct gene expression changes in peripheral blood mononuclear cells exposed to combustible versus non-combustible tobacco product preparations

    TRAN N.Q.(1); ARIMILLI S.(2); MADAHIAN B.(3); HOMAYOUNI R.(3); PRASAD G.L.(1)
    (1) RAI Services, Winston-Salem, NC, USA; (2) Wake Forest University, Winston-Salem, NC, USA; (3) Quire Inc., Memphis, TN, USA
    Cigarette smoking exerts diverse physiological effects including immune suppression. Existing epidemiological data show that consumption of smokeless tobacco products, such as moist snuff, is less harmful relative to smoking. We previously demonstrated ...
  2. CORESTA Meeting, Smoke Science/Product Technology, 2017, Kitzbühel, STPOST 19

    Combustible and smokeless tobacco product preparations differentially regulate calcium mobilization in the human leukemic HL60 cell line

    MAKENA P.(2); ARIMILLI S.(1); DAMRATOSKI B.E.(1); PRASAD G.L.(2)
    (1) Wake Forest University School of Medicine, Department of Microbiology & Immunology, Winston-Salem, NC, U.S.A.; (2) RAI Services Co., Winston-Salem, NC 27102, U.S.A.
    Increased calcium (Ca 2+) mobilization is central to leukocyte signaling and for regulation of immune responses. Although evidence suggests that cigarette smoking affects several biological functions including inflammatory responses, how the use of ...
  3. CORESTA Congress, Berlin, 2016, Smoke Science/Product Technology Groups, STPOST 40

    Role of oxidative stress in the suppression of immune responses in peripheral blood mononuclear cells exposed to combustible tobacco product preparations

    PRASAD G.L.(1); ARIMILLI S.(2); DAMRATOSKI B.E.(2)
    (1) Scientific & Regulatory Affairs, RAI Services Company, Winston-Salem, U.S.A.; (2) Department of Microbiology & Immunology, Wake Forest University Health Sciences, U.S.A.
    Cigarette smoking is a major risk factor for several human diseases. Chronic inflammation, resulting from increased oxidative stress, has been suggested as a mechanism that contributes to the increased susceptibility of smokers to cancer and microbial ...
  4. TSRC, Tob. Sci. Res. Conf., 2016, 70, abstr. 17

    Ex vivo effects of smokeless tobacco extracts on select immune responses

    PRASAD G.L.(1); ARIMILLI S.(2); DAMRATOSKI B.E.(2); SCHMIDT E.(1); ROWE J.(3); CHOUCHANE S.(4)
    (1) RAI Services Company; (2) Wake Forest University Health Sciences; (3) R.J. Reynolds Tobacco Co.; (4) Eurofins Lancaster Labs Inc, Winston-Salem, NC, USA
    Our previous work demonstrated that exposure to Tobacco Product Preparations (TPPs) from combustible tobacco cause cytotoxic and select immunosuppressive effects in PBMCs, and this ex vivo model is well suited to investigate the chronic immune suppression ...
  5. TSRC, Tob. Sci. Res. Conf., 2013, 67, abstr. 19

    Tobacco product preparations differentially regulate human PBMC functions, including T cell and NK function.

    ARIMILLI S.(1); DAMRATOSKI B.E.(1); PRASAD G.L.(2)
    (1) Wake Forest University School of Medicine, Winston-Salem, NC, USA; (2) Reynolds Tobacco Company, Winston-Salem, NC, USA
    Natural Killer (NK) cells and T cells play essential roles in innate and adaptive immune responses in protecting against microbial infections and in tumor surveillance. Although evidence suggests that smoking causes immunosuppression, there is limited ...
  6. TSRC, Tob. Sci. Res. Conf., 2012, 66, abstr. 07

    Complete artificial saliva alters expression of proinflamma-tory cytokines in human dermal fibroblasts

    MALPASS G.E.(1); ARIMILLI S.(1); PRASAD G.L.(2); HOWLETT A.C.(1)
    (1) Wake Forest University Health Sciences, Winston-Salem, NC, USA; (2) R.J. Reynolds Tobacco Company, Winston-Salem, NC, USA
    Complete artificial saliva (CAS) is a saliva substitute often used as a vehicle for test articles, including, smokeless tobacco. We discovered, using polymerase chain reaction gene expression array analyses, that CAS increased gene expression for certain ...
  7. TSRC, Tob. Sci. Res. Conf., 2012, 66, abstr. 08

    Method development for enumeration of endothelial progenitor cells (EPCs)

    PRASAD G.L.(1); ARIMILLI S.(2); JONES B.A.(1); FEARON I.(3)
    (1) R.J. Reynolds Tobacco Company, Winston-Salem, NC, USA; (2) Wake Forest University Baptist Health, Winston-Salem, NC, USA; (3) British American Tobacco GR&D, Southampton, UK
    Endothelial progenitor cells (EPCs) are a type of white blood cells present in circulation at low numbers and are hypothesized to play a role in the protection and repair of the of cardiovascular system. EPCs, characterized as cells expressing the surface ...
  8. TSRC, Tob. Sci. Res. Conf., 2011, 65, abstr. 05

    A cell culture model for chronic exposure to cigarette smoke.

    ARIMILLI S.(1); DAMRATOSKI B.E.(1); PRASAD G.L.(2)
    (1) Dept. of Microbiology & Immunology, Wake Forest University School of Medicine, Winston Salem, NC, USA; (2) R.J. Reynolds Tobacco Company, Winston Salem, NC, USA
    A wide range of experimental models, including cell culture systems, have been developed to investigate the cytotoxic and biological effects of cigarette smoke and its constituents, individually and by chemical class. For example, cell culture studies ...
  9. TSRC, Tob. Sci. Res. Conf., 2010, 64, abstr. 19

    Optimization of leukocyte isolation for clinical studies

    ARIMILLI S.; DAMRATOSKI B.E.; CHEN P.; JONES B.A.; SWORDS W.E.; PRASAD G.L.
    Wake Forest University School of Medicine, Dept. of Microbiology & Immunology, Winston-Salem, NC, USA; R.J. Reynolds Tobacco Co., IR&D Dept., Winston-Salem, NC, USA
    Isolation and processing blood into its constituent cell types is an important process in basic and clinical research. Although a number of reagents are available and methods have been developed to fractionate blood, there is a need to optimize methods ...
  10. TSRC, Tob. Sci. Res. Conf., 2010, 64, abstr. 22

    Evaluation of cytotoxicity of different tobacco preparations

    ARIMILLI S.; DAMRATOSKI B.E.; BOMBICK B.; SWORDS W.E.; BORGERDING M.; PRASAD G.L.
    Wake Forest University School of Medicine, Dept. of Microbiology & Immunology, Winston-Salem, NC, USA
    Current debate regarding the potential for significant reductions in human health risk associated with smokeless tobacco (ST) use compared to cigarette smoking suggests further examination of the relative cellular/molecular effects of non-combustible and ...