Search Results

18 results

  1. TSRC, Tob. Sci. Res. Conf., 2018, 72, abstr. 111 (also presented at CORESTA 2018)

    Characterization and certification of smokeless tobacco reference products

    SLONE S.; CANETE S.; JI Huihua; JACK A.M.; BUSH L.P.; CHAMBERS O.; SHELTON B.
    University of Kentucky, Lexington, KY, USA
    The constituents of raw tobacco products are naturally variable, and product design, the manufacturing process and the evolving science of tobacco analytics may increase this variability. The call for certified tobacco reference materials and their ...
  2. CORESTA Meeting, Smoke Science/Product Technology, 2017, Kitzbühel, STPOST 45 (also presented at TSRC 2017)

    Stability of the certified 1R6F reference cigarette

    JI H.(1); WU Y.(1); FANNIN F.F.(2); BUSH L.P.(2)
    (1) University of Kentucky, Center of Tobacco Reference Products, Lexington, KY, U.S.A.; (2) University of Kentucky, Department of Plant and Soil Sciences, Lexington, KY, U.S.A.
    University of Kentucky Center of Tobacco Reference Products (CTRP) has provided reference cigarettes for almost 50 years. These reference cigarettes are widely used in tobacco research including analytical method development and modified risk tobacco ...
  3. 47th TWC, Tob. Work. Conf., 2016, abstr. 16

    Effect of artificial ordering on TSNAs in burley tobacco during short term bulking between takedown and stripping

    FISHER C.R.; JACK A.M.; BUSH L.P.; PEARCE R.
    University of Kentucky, Lexington KY USA
    Production guidelines caution burley tobacco growers against using water sprays to bring tobacco into case and storing high-moisture tobacco because of concerns that these practices can lead to the accumulation of TSNAs. A series of experiments over three ...
  4. CORESTA Congress, Quebec, 2014, Smoke Science/Product Technology Groups, ST 88

    Quantitative determination of enantiomers of tobacco-specific nitrosamines in tobacco using gas chromatography-mass spectrometry

    JI H.(1); FANNIN F.(2); CAI B.(2); BUSH L.P.(2)
    (1) University of Kentucky, Kentucky Tobacco Research and Development Center, Lexington, KY, U.S.A.; (2) University of Kentucky, Department of Plant and Soil Sciences, Lexington, KY, U.S.A.
    Different enantiomers of tobacco-specific nitrosamines (TSNAs) have different biological activities. TSNAs exist as either (R)- or (S)-enantiomeric isoforms that differ at the 2'-C position of the pyrrolidine ring. It has been reported that (S)-N& ...
  5. TSRC, Tob. Sci. Res. Conf., 2013, 67, abstr. 43

    The pattern of benzo[a]pyrene and tobacco-specific nitrosamine accumulation in fire-cured tobacco

    JACK A.M.; BAILEY W.A.; SCHOERGENDORFER A.; JI H.; SINGLETON J.; FANNIN N.; BUSH L.P.
    University of Kentucky, Lexington, KY, USA
    The accumulation of benzo[a]pyrene (B[a]P) and tobacco specific nitrosamines (TSNAs) during fire-curing was studied in order to establish whether B[a]P and TSNAs can be reduced by modifying the firing regime. The dark fire-cured variety PD 7309 was grown ...
  6. 45th TWC, Tob. Work. Conf., 2012, abstr. 42

    Selection for the low alkaloid trait: Part 1 - phenotypic selection

    JACK A.M.; FANNIN F.F.; JI H.; YANG W.; BUSH L.P.
    Kentucky Tobacco Research & Development Center, University of Kentucky, 322 Plant Science Building, Lexington, KY 40546 USA
    The objective of this study was to find the best sampling stage for phenotypic selection of low alkaloid genotypes. Traditionally, breeders sampled mature plants in which the alkaloids were higher and could be measured more easily. However, with modern ...
  7. 45th TWC, Tob. Work. Conf., 2012, abstr. 88

    Microbial endophytes and TSNA accumulation: some preliminary results

    FISHER C.R.; MOE L.; JACK A.M.; BUSH L.P.
    Plant and Soil Sciences Dept., University of Kentucky, 317 Plant Science Building, Lexington, KY 40546 USA
    Tobacco-specific nitrosamine (TSNA) accumulation during burley curing is probably driven by endophytic microbial anaerobic denitrification. This study investigates the effect of time of spraying a soil suspension onto greenhouse-grown plants that ...
  8. TSRC, Tob. Sci. Res. Conf., 2012, 66, abstr. 13

    Accumulation of nicotine and nornicotine enantiomers in leaf of nicotine demethylases mutants during growth and curing

    CAI B.(1); JACK A.M.(1); LEWIS R.S.(2); DEWEY R.E.(2); JI H.(1); BUSH L.P.(1)
    (1) University of Kentucky, Lexington, KY, USA; (2) North Carolina State University, Raleigh, NC, USA
    Nicotine metabolism in tobacco plants is mostly through N-demethylation, resulting in nornicotine. There are three functional nicotine demethylases CYP82E4 (E4), CYP82E5v2 (E5) and CYP82E10 (E10) in tobacco. In this study, we investigated the nicotine and ...