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  1. Tobacco Science & Technology, 2010, 7, p. 48-52., ISSN.1002-0861

    SRAP analysis of D4 lines of Purslane DNA-introduced tobacco

    DAI LIN-JIAN(1); XU SHUANG-HONG(2); SUN HUAN-LIANG(1); WANG KUN(1); ZHONG JUN(1)
    1. College of Agronomy, Hunan Agricultural University, Changsha 410128, P.R. China; 2. China Tobacco Hunan Industrial Co., Ltd., Changsha 410128, P.R. China
    By introducing purslane DNA into tobacco via pollen-tube pathway, the 4th generation self-bred progeny (D4) was obtained, and the purity of 32 selected D4 were analyzed. The results showed that the optimum SRAP-PCR reaction system includes a solution of ...