Tobacco Science & Technology, 2010, 8, p. 34-40., ISSN.1002-0861
Determination of twenty free amino acids in tobacco leaves by HPLC with pre-column derivatization
Shenzhen Boton Flavours and Fragrances Co., Ltd, Shenzhen 518051, Guangdong, P.R. China
A method for determining 20 free amino acids in tobacco leaf was established by using 2,4-dinitrofluorobenzene (DNFB) as a derivative reagent, 0.015 mol/L disodium hydrogen phosphate and potassium dihydrogen phosphate as buffer solution and 50% aqueous ...