Search Results

65 results

  1. CORESTA Meeting, Agronomy/Phytopathology, Rovinj, 2009, AP 17

    Characterization of TSNA content in cured leaf of dark fire-cured and dark air-cured tobaccos grown in KY and TN, U.S.A. in 2006-2008

    NIELSEN M.T.; BENNETT C.B.; FUFA S.; HELTSLEY D.; FINCH J.
    U.S. Smokeless Tobacco Manufacturing Co., Nashville, TN, USA
    Tobacco specific nitrosamines (TSNA's) found in the cured leaf of dark tobaccos are formed by the nitrosation of secondary amine alkaloids during post-harvest stages of leaf production. While previous research has identified many factors, including ...
  2. CORESTA Congress, Shanghai, 2008, AP 05

    Down-regulating the nicotine demethylase gene lowers NNN content levels with little or no effect on field performance of Burley and dark tobaccos

    NIELSEN M.; XU Dongmei; LUSSO M.; DAVIS G.; SHEN Yanxin; BAILEY A.; HART F.; CUI Mingwu; NORMAN D.
    U.S. Smokeless Tobacco Co., Winchester, KY, USA
    Down-regulating nicotine demethylase (ND) activity in tobacco using RNAi interference mechanisms or by mutating the ND gene reduces nornicotine formation and should lead to lowering N-nitroso-nornicotine (NNN) levels in cured leaf. Over the past two years ...
  3. CORESTA Congress, Shanghai, 2008, AP 06

    Reducing nicotine conversion: stability of RNAi-mediated and mutant down-regulation of nicotine demethylase

    XU Dongmei; SHEN Yanxin; LUSSO M.; BAILEY A.; NORMAN D.; DAVIS G.; HART F.; CUI Mingwu; NIELSEN M.
    U.S. Smokeless Tobacco Co., Winchester, KY, USA
    Nornicotine, a secondary alkaloid formed from the demethylation of nicotine in tobacco, is the precursor of N'-nitrosonornicotine (NNN), a TSNA formed by nitrosation during tobacco curing. In earlier studies, we reported that GMO (RNAi) and non-GMO ...
  4. TSRC, Tob. Sci. Res. Conf., 2008, 62, abstr. 29

    Productivity improvements through chromatography and automation for GC/TEA TSNA analysis of tobacco leaf

    HARRIS W.E.; GIBSON S.; FINCH J.N.
    U.S. Smokeless Tobacco-Manufacturing Co., Nashville, TN, USA
    To improve productivity of TSNA analysis in the laboratory by GC/TEA, an automated sample handling/extraction system coupled with micro-bore chromatography was developed and implemented. The goal of the development system was to be able to analyze 80+ ...
  5. Beitr. Tabakforsch. Int./Contrib. Tob. Research, 2007, 22-5, p. 358-64., ISSN.0173-783X

    A model for the prediction of tobacco temperature and oxygen profiles in warehouse aging process

    ZHEN Y.; CHIPLEY J.; DOW A.
    U.S. Smokeless Tobacco Inc, Nashville, TN, USA
    A mathematical model on the temperature and oxygen profiles for the tobacco warehouse aging process was formulated and solved by numeric analysis. The mode parametres were obtained using the non-linear regression method by fitting several years measured ...