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4 results

  1. CORESTA Congress, Shanghai, 2008, AP 05

    Down-regulating the nicotine demethylase gene lowers NNN content levels with little or no effect on field performance of Burley and dark tobaccos

    NIELSEN M.; XU Dongmei; LUSSO M.; DAVIS G.; SHEN Yanxin; BAILEY A.; HART F.; CUI Mingwu; NORMAN D.
    U.S. Smokeless Tobacco Co., Winchester, KY, USA
    Down-regulating nicotine demethylase (ND) activity in tobacco using RNAi interference mechanisms or by mutating the ND gene reduces nornicotine formation and should lead to lowering N-nitroso-nornicotine (NNN) levels in cured leaf. Over the past two years ...
  2. CORESTA Congress, Shanghai, 2008, AP 06

    Reducing nicotine conversion: stability of RNAi-mediated and mutant down-regulation of nicotine demethylase

    XU Dongmei; SHEN Yanxin; LUSSO M.; BAILEY A.; NORMAN D.; DAVIS G.; HART F.; CUI Mingwu; NIELSEN M.
    U.S. Smokeless Tobacco Co., Winchester, KY, USA
    Nornicotine, a secondary alkaloid formed from the demethylation of nicotine in tobacco, is the precursor of N'-nitrosonornicotine (NNN), a TSNA formed by nitrosation during tobacco curing. In earlier studies, we reported that GMO (RNAi) and non-GMO ...