Search Results

35 results

  1. CORESTA Congress, Kunming, 2018, Smoke Science/Product Technology Groups, STPOST 43

    In vitro micronucleus (MN) assay using TK6 cells: review of historical positive and negative control data

    STANKOWSKI L.F. Jr(1); HURTADO S.B.(1); WELLS M.M.(1); KWOK V.Y.(1); DOSHI U.(2); COFFA B.G.(2); LEE K.M.(2)
    (1) Charles River Laboratories, Genetic and In Vitro Toxicology, Skokie, IL, U.S.A.; (2) Altria Client Services LLC, Research, Development & Regulatory Affairs, Richmond, VA, U.S.A.
    TK6 human lymphoblastoid cells are widely used in the in vitro MN assay as a part of regulatory genotoxicity testing for pharmaceuticals, but have seen relatively limited use in testing of other test materials, including tobacco-related products. As an ...
  2. CORESTA Congress, Kunming, 2018, Smoke Science/Product Technology Groups, STPOST 46

    Validation of an in vitro test battery to evaluate smokeless tobacco products

    HURTADO S.B.(1); LALLIER B.(1); TYDRICK C.(1); LIU Z.(2); GUMP J.(2); BISWAS K.(2); KWOK V.Y.(1); STANKOWSKI L.F. Jr(1)
    (1) Charles River Laboratories, Genetic and In Vitro Toxicology, Skokie, IL, U.S.A.; (2) Charles River Laboratories, Analytical Chemistry, Ashland, OH, U.S.A.
    Evaluating potential cytotoxicity and genotoxicity of smokeless tobacco products poses unique challenges for in vitro testing. Each product is a complex mixture of vegetable matter, and may include a packaging component as part of the final product. ...
  3. CORESTA Meeting, Smoke Science/Product Technology, 2017, Kitzbühel, STPOST 44

    Validation and comparison of the neutral red uptake assay in BALB/c 3T3 and CHO-WBL cells

    HURTADO S.B; CALLUPE J.; MARTINEZ J.L.; SOOMER-JAMES J.; GROMER K.D.; KWOK V.Y.; FARABAUGH C.S.; STANKOWSKI L.F. Jr.
    Charles River Laboratories - Skokie, Skokie, IL, U.S.A.
    The neutral red uptake (NRU) assay is used to evaluate the cytotoxicity of a variety of chemicals, cigarette smoke fractions, e-liquids, medical devices, etc. in vitro. Charles River Laboratories-Skokie previously validated the NRU assay in BALB/c 3T3 ...
  4. CORESTA Meeting, Smoke Science/Product Technology, 2017, Kitzbühel, STPOST 46

    Validation and comparison of the in vitro micronucleus assay in TK6 and CHO-WBL cells

    WELLS M.; LORENZ M.; SOOMER-JAMES J.; KAYE S.L.; GROMER K.D.; BHATTACHARYA R.; OCO K.R.; KWOK V.Y.; FARABAUGH C.S.; STANKOWSKI L.F. Jr.
    Charles River Laboratories - Skokie, Skokie, IL, U.S.A.
    The in vitro micronucleus (MN) assay is used to evaluate the potential clastogenicity and aneugenicity of a variety of chemicals and agents in vitro. Charles River Laboratories-Skokie previously validated the in vitro MN assay in TK6 cells, according to ...
  5. CORESTA Congress, Berlin, 2016, Smoke Science/Product Technology Groups, STPOST 21 (also presented at TSRC 2016)

    Puff-by-puff tar, nicotine and carbonyl profiles under different smoking regimens of commercial cigarettes with various carbon filter technologies

    ROBERTSON R.M.; NORFLEET K.; CANTERBURY W.B.
    Celanese, Narrows, VA, U.S.A.
    Puff-by-puff smoke analysis has shown that the CelFX ® carbon filter technology has similar nicotine and tar smoke delivery profiles but with significantly reduced carbonyls, particularly in the first 6 puffs, compared to a cellulose acetate filter. Prior ...
  6. TSRC, Tob. Sci. Res. Conf., 2016, 70, abstr. 14 (also presented at CORESTA 2016)

    Puff-by-puff tar, nicotine and carbonyl profiles under different smoking regimens of commercial cigarettes with various carbon filter technologies

    ROBERTSON R.M.
    Celanese, Narrows, VA, USA
    Puff by puff smoke analysis has shown that the CelFX ® carbon filter technology has similar nicotine and tar smoke delivery profiles but with significantly reduced carbonyls, particularly in the first 6 puffs, compared to a cellulose acetate filter. Prior ...
  7. CORESTA Meeting, Smoke Science/Product Technology, 2015, Jeju, STPOST 18

    Particle breakthrough comparison of commercial carbon filters with CelFX™ carbon/acetate filters

    ROBERTSON R.M.; SUTHAR J.N.; BASU S.
    Celanese, Narrows, VA, U.S.A.
    Previous works have evaluated cigarette filter carbon particle release from various filter designs. The recently introduced CelFX™ Matrix Technology offers high loadings of carbon, with low pressure drops and has not been studied for the particle release ...